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Alternation of cytosolic carbonic anhydrase isoenzymes during deciduomatal development in pregnant mice  Whei-Ling Chiang, Ph.D., Jer-Yuh Liu, Ph.D.,

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Presentation on theme: "Alternation of cytosolic carbonic anhydrase isoenzymes during deciduomatal development in pregnant mice  Whei-Ling Chiang, Ph.D., Jer-Yuh Liu, Ph.D.,"— Presentation transcript:

1 Alternation of cytosolic carbonic anhydrase isoenzymes during deciduomatal development in pregnant mice  Whei-Ling Chiang, Ph.D., Jer-Yuh Liu, Ph.D., Chu-Yu Liao, M.S., Shun-Fa Yang, M.S., Yih-Shou Hsieh, Ph.D., Shu-Chen Chu, Ph.D.  Fertility and Sterility  Volume 82, Pages (October 2004) DOI: /j.fertnstert Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

2 FIGURE 1 The expression of carbonic anhydrases (CAs) in decidualized uterine tissue of mice on days 0.5–10.5 after mating. The uterine tissues homogenate was prepared and subjected to CA activity assay, immunoblotting analysis (with α-tubulin as an internal control), and reverse transcriptase-polymerase chain reaction (RT-PCR) (with glyceraldehyde-3-phosphate-dehydrogenase [GAPDH] as an internal control). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

3 FIGURE 2 Number of embryos in decidualized uterine tissue of mice from various groups on day 2.5 after mating. ***P<.001. SP = substance P; CAi = carbonic anhydrase inhibitor. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

4 FIGURE 3 Analysis of the carbonic anhydrase (CA) protein level in decidualized uterine tissue of mice from four groups on day 2.5 after mating. The uterine tissues homogenate was prepared and subjected to immunoblotting analysis for CA I, CA II, and CA III. Here, α-tubulin was used as a control. SP = substance P; CAi = carbonic anhydrase inhibitor. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions

5 FIGURE 4 Relative carbonic anhydrase (CA) protein level was quantified by densitometry in decidualized uterine tissue of mice from four groups (CA activity: dark red; CA I: cyan; CA II: dark blue; CA III: dark green) on day 2.5 after mating. *P<.05; **P<.01; ***P<.001. SP = substance P; CAi = carbonic anhydrase inhibitor. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions


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