Presentation is loading. Please wait.

Presentation is loading. Please wait.

Phenotypic and Functional Outcome of Human Monocytes or Monocyte-Derived Dendritic Cells in a Dermal Equivalent  G. Guironnet, C. Dezutter-Dambuyant,

Published byEugenia Neal Modified over 5 years ago

Similar presentations

Presentation on theme: "Phenotypic and Functional Outcome of Human Monocytes or Monocyte-Derived Dendritic Cells in a Dermal Equivalent  G. Guironnet, C. Dezutter-Dambuyant,"— Presentation transcript:

1 Phenotypic and Functional Outcome of Human Monocytes or Monocyte-Derived Dendritic Cells in a Dermal Equivalent  G. Guironnet, C. Dezutter-Dambuyant, A. Gaudillère, S. Maréchal, D. Schmitt, J. Péguet-Navarro  Journal of Investigative Dermatology  Volume 116, Issue 6, Pages (June 2001) DOI: /j x Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Flow cytometry analysis of the cells before incorporation into the lattices. Freshly isolated monocytes and day 6 monocyte-derived DC cultured in either GM-CSF/IL-4 (DC) or GM-CSF/IL-4/TGF-β1 (TGF-DC) were stained with different monoclonal antibodies (closed histograms). Cytometry analysis was performed without gating. Open histograms give the reactivity with the appropriate isotype-matched control monoclonal antibodies. Data are from one representative experiment out of 20 routinely executed. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Immunostaining of lattice cryosections after incorporation of GM-CSF/IL-4-derived DC. DC were seeded in dermal equivalents and cultured for 7 d without cytokines or in the presence of exogenous GM-CSF/IL-4 (+ cytokines). Four micron lattice cryosections were stained using control Ig, HLA-DR, CD14, CD1a, CD68, and FXIIIa and were observed by fluorescence microscopy. Scale bar, 50 µm. Results are representative of three experiments. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 Double staining and flow cytometric analysis of cells recovered from the lattices. Monocytes, DC, or TGF-DC were seeded into lattices and cultured for 7 d in the presence or not (+0) of exogenous cytokines. Lattices were then digested with collagenase and the isolated cells were double stained using anti-HLA-DR-tri-color (y axis) and anti-CD14-FITC or anti-CD1a-FITC (x axis). Quadrant setting was performed according to the reactivities of isotype-matched control monoclonal antibodies. Results are representative of at least five experiments. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 Allostimulatory property of the cells isolated from the lattices. Monocytes (a), GM-CSF/IL-4-derived DC (DC) (b), or GM-CSF/IL-4/TGF-β1-derived DC (TGF-DC) (c) were seeded into lattices and cultured for 7 d with or without the exogenous cytokines (filled squares and open circles, respectively). Lattices were then digested and, after irradiation, the recovered cells were added to allogeneic T cells in an MLR assay. The stimulator cell number corresponds to the number of HLA-DR+ cells in the dermal suspensions, as assessed by flow cytometry analysis. As negative controls, freshly isolated monocytes were tested in MLR (filled triangles). All the tests were carried out in triplicate and T cell proliferation was assessed by 3H-thymidine incorporation during the last 18 h of culture. Results are expressed as mean cpm ± SD and are representative of three independent experiments. The cpm value for T cells alone or DC alone did not exceed 40 cpm. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Electron microscopy analysis of the cells inside the lattices. DC (1–2) or TGF-DC (3–4) were seeded in the dermal equivalents and cultured for 7 d with (1–3) or without (2–4) the exogenous cytokines. Lattices were then processed for electron microscopy analysis. Ultrathin sections were examined on a JEOL 1200 EX electron microscope. Inserts: endocytic structures. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2001 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Phenotypic and Functional Outcome of Human Monocytes or Monocyte-Derived Dendritic Cells in a Dermal Equivalent  G. Guironnet, C. Dezutter-Dambuyant,"

Similar presentations

Chemokine Receptor Expression on Neoplastic and Reactive T Cells in the Skin at Different Stages of Mycosis Fungoides  Tilmann Kallinich, J. Marcus Muche,

Chemokine Receptor Expression on Neoplastic and Reactive T Cells in the Skin at Different Stages of Mycosis Fungoides  Tilmann Kallinich, J. Marcus Muche,
Functional Expression and Analysis of a Human HLA-DQ Restricted, Nickel-Reactive T Cell Receptor in Mouse Hybridoma Cells  Jörg Vollmer, Hans Ulrich Weltzien,

Functional Expression and Analysis of a Human HLA-DQ Restricted, Nickel-Reactive T Cell Receptor in Mouse Hybridoma Cells  Jörg Vollmer, Hans Ulrich Weltzien,
In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells  Martine A.

In situ Delivery of Tumor Antigen– and Adjuvant-Loaded Liposomes Boosts Antigen- Specific T-Cell Responses by Human Dermal Dendritic Cells  Martine A.
Volume 49, Issue 1, Pages (January 2006)

Volume 49, Issue 1, Pages (January 2006)
Non-small Cell Lung Cancer Induces an Immunosuppressive Phenotype of Dendritic Cells in Tumor Microenvironment by Upregulating B7-H3  Thomas Schneider,

Non-small Cell Lung Cancer Induces an Immunosuppressive Phenotype of Dendritic Cells in Tumor Microenvironment by Upregulating B7-H3  Thomas Schneider,
UV Radiation Induces the Epidermal Recruitment of Dendritic Cells that Compensate for the Depletion of Langerhans Cells in Human Skin  Amine Achachi,

UV Radiation Induces the Epidermal Recruitment of Dendritic Cells that Compensate for the Depletion of Langerhans Cells in Human Skin  Amine Achachi,
Tatsukuni Ohno, Yuta Kondo, Chenyang Zhang, Siwen Kang, Miyuki Azuma 

Tatsukuni Ohno, Yuta Kondo, Chenyang Zhang, Siwen Kang, Miyuki Azuma 
Volume 135, Issue 6, Pages (December 2008)

Volume 135, Issue 6, Pages (December 2008)
Fibroblast Activation Protein: Differential Expression and Serine Protease Activity in Reactive Stromal Fibroblasts of Melanocytic Skin Tumors  Margit.

Fibroblast Activation Protein: Differential Expression and Serine Protease Activity in Reactive Stromal Fibroblasts of Melanocytic Skin Tumors  Margit.
CD90+ Human Dermal Stromal Cells Are Potent Inducers of FoxP3+ Regulatory T Cells  Karin Pfisterer, Karoline M. Lipnik, Erhard Hofer, Adelheid Elbe-Bürger 

CD90+ Human Dermal Stromal Cells Are Potent Inducers of FoxP3+ Regulatory T Cells  Karin Pfisterer, Karoline M. Lipnik, Erhard Hofer, Adelheid Elbe-Bürger 
Ex vivo induction of multiple myeloma–specific cytotoxic T lymphocytes

Ex vivo induction of multiple myeloma–specific cytotoxic T lymphocytes
Gianni Gerlini, Hans Peter Hefti, Martin Kleinhans, Brian J

Gianni Gerlini, Hans Peter Hefti, Martin Kleinhans, Brian J
Expansion of FOXP3high regulatory T cells by human dendritic cells (DCs) in vitro and after injection of cytokine-matured DCs in myeloma patients by Devi.

Expansion of FOXP3high regulatory T cells by human dendritic cells (DCs) in vitro and after injection of cytokine-matured DCs in myeloma patients by Devi.
Cord Blood CD34+ Cells Differentiate into Dermal Dendritic Cells in Co-Culture with Cutaneous Fibroblasts or Stromal Cells  Zia U.A. Mollah, Setsuya Aiba,

Cord Blood CD34+ Cells Differentiate into Dermal Dendritic Cells in Co-Culture with Cutaneous Fibroblasts or Stromal Cells  Zia U.A. Mollah, Setsuya Aiba,
IC3b Arrests Monocytic Cell Differentiation Into CD1c-Expressing Dendritic Cell Precursors: A Mechanism for Transiently Decreased Dendritic Cells in vivo.

IC3b Arrests Monocytic Cell Differentiation Into CD1c-Expressing Dendritic Cell Precursors: A Mechanism for Transiently Decreased Dendritic Cells in vivo.
Bifurcated Dendritic Cell Differentiation In Vitro From Murine Lineage Phenotype-Negative c-kit + Bone Marrow Hematopoietic Progenitor Cells by Yi Zhang,

Bifurcated Dendritic Cell Differentiation In Vitro From Murine Lineage Phenotype-Negative c-kit + Bone Marrow Hematopoietic Progenitor Cells by Yi Zhang,
PD-1 on Immature and PD-1 Ligands on Migratory Human Langerhans Cells Regulate Antigen-Presenting Cell Activity  Victor Peña-Cruz, Sean M. McDonough,

PD-1 on Immature and PD-1 Ligands on Migratory Human Langerhans Cells Regulate Antigen-Presenting Cell Activity  Victor Peña-Cruz, Sean M. McDonough,
Myeloid-Derived Suppressor Cells in Psoriasis Are an Expanded Population Exhibiting Diverse T-Cell–Suppressor Mechanisms  Lauren Y. Cao, Jin-Sung Chung,

Myeloid-Derived Suppressor Cells in Psoriasis Are an Expanded Population Exhibiting Diverse T-Cell–Suppressor Mechanisms  Lauren Y. Cao, Jin-Sung Chung,
Characterization of Interleukin-17–Producing Regulatory T Cells in Inflamed Intestinal Mucosa From Patients With Inflammatory Bowel Diseases  Zaruhi Hovhannisyan,

Characterization of Interleukin-17–Producing Regulatory T Cells in Inflamed Intestinal Mucosa From Patients With Inflammatory Bowel Diseases  Zaruhi Hovhannisyan,
TGF-β combined with M-CSF and IL-4 induces generation of immune inhibitory cord blood dendritic cells capable of enhancing cytokine-induced ex vivo expansion.

TGF-β combined with M-CSF and IL-4 induces generation of immune inhibitory cord blood dendritic cells capable of enhancing cytokine-induced ex vivo expansion.

Similar presentations

Ads by Google