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A B 5 DAF 7 DAF 9 DAF 11 DAF 13 DAF Reference 5 DAF 7 DAF 9 DAF 11 DAF

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Presentation on theme: "A B 5 DAF 7 DAF 9 DAF 11 DAF 13 DAF Reference 5 DAF 7 DAF 9 DAF 11 DAF"— Presentation transcript:

1 A B 5 DAF 7 DAF 9 DAF 11 DAF 13 DAF Reference 5 DAF 7 DAF 9 DAF 11 DAF
pI kD 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 5 DAF 7 DAF 9 DAF 116 97 84 66 55 45 36 24 29 20 pI 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 kD 116 11 DAF 13 DAF Reference 84 97 66 55 45 36 29 24 20 B pI kD 4.0 5.0 6.0 7.0 116 5 DAF 7 DAF 9 DAF 97 84 66 55 45 36 29 24 20 pI 4.0 5.0 6.0 7.0 kD 116 11 DAF 13 DAF Reference 97 84 66 55 45 36 29 24 20 Supplemental Figure 1. Two-dimensional electrophoretic (2-DE) analysis of proteins (50 g) isolated from immature seeds of 5, 7, 9, 11, or 13 days after flowering (DAF) and labeled with n-hydroxysuccinimide-activated Cy5. Approximately 200 seeds from each of the five individual stages were pooled in biological triplicate and proteins isolated for two analytical and two reference gels (pH 3-10 and 4-7) per biological replicate. A, 2-DE analysis of developing seed proteins using pH 3 to 10 wide-range IPG strips. B, 2-DE analysis of developing seed proteins using pH 4 to 7 medium-range IPG strips. Gels are representative of the three biological replicates that were analyzed. The reference gel is a composite of all five stages obtained by pooling of 10 mg of protein from all five seed stages prior to labeling.

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