1. Products > ZR-75-1 Transfection Reagent (Breast Carcinoma, CRL1500)
Altogen labs
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > ZR-75-1 Transfection Reagent (Breast Carcinoma, CRL1500)
Altogen Biosystems offers the ZR-75-1 Transfection Reagent among a host of 100+ cell line specific In Vitro Transfection Kits.
The ZR-75-1 Transfection Reagent is a proprietary cationic lipids formulation, and it has been developed to provide high transfection efficiency with the ZR-75-1 cell line.
This cell line is a good host for studying breast cancer. When cultured in vitro, ZR-75-1 cells are a monolayer and adhere to the surface of the culture flask.
Other applications include drug discovery, gene expression studies, molecular and cell biology research applications.
Purchase ZR-75-1 Transfection Kit at
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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2. High Density ZR-75-1 cells
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > ZR-75-1 Transfection Reagent (Breast Carcinoma, CRL1500)
ZR-75-1 Cell Line Description and Applications
The ZR-75-1 cell line originates from the ductal carcinoma of the mammary gland of a 63-year old female patient. This cell line is epithelial and has a population doubling time of approximately 80 hours. ZR-75-1 cells have a hypertriploid chromosome number, express estrogen receptors and produce high levels of MUC-1 mucin mRNA. The modal chromosome number of this cell line is 72. The ZR-75-1 cell line is useful as a tool for studying tumor properties, but it is also particularly helpful in studying breast cancer.
High Density ZR-75-1 cells
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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3. ZR-75-1 Transfection Protocol
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > ZR-75-1 Transfection Reagent (Breast Carcinoma, CRL1500)
ZR-75-1 Transfection Protocol
1. Plate 10, ,000 ZR-75-1 cells per well in 0.5 ml of complete growth medium 12–24 hours prior to transfection
2. Wash with 1xPBS and add 0.5 ml of fresh growth medium
3. Prepare transfection complexes by mixing 40 µl of serum-free medium, 5.5 µl of transfection reagent, and
• 750 ng DNA (or mRNA), or
• 30 nM - 50 nM of siRNA (or microRNA)
*Referred to a final volume including growth medium
4. Incubate transfection complexes at RT for minutes
5. Optional: Add 2 µl of Complex Condenser. This reagent reduces the size of transfection complex, therefore increasing transfection efficiency; however, it may increase cell toxicity
6. Add prepared transfection complexes to 0.5 ml of complete growth medium with ZR-75-1 cells (from step 2)
7. Incubate cells at 37ºC in a humidified CO2 incubator
8. Assay for phenotype or target gene expression hours after transfection
Optional: Transfection efficiency can be increased by addition of Transfection Enhancer reagent. Add 2 µl of Transfection Enhancer reagent hours after transfection
If the viability of ZR-75-1 cells being transfected is affected at hours post-transfection, the level of cytotoxicity can be decreased by changing the growth medium and eliminating redundant exposure of cells to transfectant
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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4. General Lipoplex-mediated Transfection Mechanism of Action
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > ZR-75-1 Transfection Reagent (Breast Carcinoma, CRL1500)
General Lipoplex-mediated Transfection Mechanism of Action
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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5. ZR-75-1 Transfection Kit Product Details
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > ZR-75-1 Transfection Reagent (Breast Carcinoma, CRL1500)
ZR-75-1 Transfection Kit Product Details
Proprietary cationic lipids formulation
Optimized for intracellular delivery of plasmid DNA, siRNA, microRNA, and mRNA
High transfection efficiency of both siRNA and plasmid DNA without compromising cell viability
Achieve robust siRNA uptake for dependable gene silencing
Effective transfection under conditions of up to 40% serum
Transfection kit includes Transfection Enhancer reagent
Gentle enough to be used for single cell analysis
Developed and manufactured by Altogen Biosystems (
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
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6. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > ZR-75-1 Transfection Reagent (Breast Carcinoma, CRL1500)
Data
Figure 1. Cyclophilin B silencing efficiency was determined by qRT-PCR in the ZR75 cells transfected by Cyclophilin B siRNA or non-silencing siRNA control following the recommended transfection protocol. Cyclophilin mRNA expression levels were measured 48 hours post-transfection. 18S rRNA levels were used to normalize the Cyclophilin B data. Values are normalized to untreated sample. Data are presented as means ± SD (n=6).

7. Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > ZR-75-1 Transfection Reagent (Breast Carcinoma, CRL1500)
Data
Figure 2. Protein expression of Cyclophilin B in ZR-75-1 cells. DNA plasmid expressing Cyclophilin B or siRNA targeting Cyclophilin B were transfected into ZR-75-1 cells following Altogen Biosystems transfection protocol. At 72 hours post-transfection the cells were analyzed by Western Blot for protein expression levels (normalized by total protein, 10 µg of total protein loaded per each well). Untreated cells used as a negative control.

8. ZR-75-1 Transfection Kit Benefits
Leading Developer and Manufacturer of In Vivo and DNA Transfection Kits, Transfection Reagents and Electroporation Delivery Products
Products > ZR-75-1 Transfection Reagent (Breast Carcinoma, CRL1500)
ZR-75-1 Transfection Kit Benefits
Pre-optimized transfection protocol for ZR-75-1 cell line
Compatible with DNA, small RNA (siRNA, shRNA, miRNA), mRNA, and small protein complexing
Free of serum and protein of animal origin
Compatible with standard and reverse transfection methods (both protocols provided in the kit manual)
Easy to use ZR-75-1 transfection protocol ensures great performance with expedited experimental timeline
Equally efficient for single or multiple transfections
Can be used for transient transfection and development of stable ZR-75-1 cell lines
Bio-degradable after endocytosis
Used for preclinical research worldwide
Altogen Biosystems  848 Rainbow Blvd #823  Las Vegas  NV   USA
Telephone   Fax   