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Effects of clonidine on lidocaine-induced inhibition of axonal transport in cultured mouse dorsal root ganglion neurones  H. Hiruma, K. Shimizu, T. Takenami,

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Presentation on theme: "Effects of clonidine on lidocaine-induced inhibition of axonal transport in cultured mouse dorsal root ganglion neurones  H. Hiruma, K. Shimizu, T. Takenami,"— Presentation transcript:

1 Effects of clonidine on lidocaine-induced inhibition of axonal transport in cultured mouse dorsal root ganglion neurones  H. Hiruma, K. Shimizu, T. Takenami, H. Sugie, T. Kawakami  British Journal of Anaesthesia  Volume 101, Issue 5, Pages (November 2008) DOI: /bja/aen265 Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

2 Fig 1 Schematic representation of the experimental chamber and the video microscopy recording system. Scale bar indicates 2 μm. British Journal of Anaesthesia  , DOI: ( /bja/aen265) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

3 Fig 2 Changes in axonal transport of organelles before and during treatment with PSS (a), 10 μM clonidine (b), 100 μM lidocaine (c), a combination of 10 μM clonidine and 100 μM lidocaine (d), and a combination of 10 μM yohimbine, 10 μM clonidine, and 100 μM lidocaine (e). The number of organelles moving in anterograde and retrograde directions was counted for 1 min at 3 min intervals by video microscopy. Horizontal axis indicates the time from the start of the treatment period (indicated by horizontal bar). Data are expressed as mean (sd) of five experiments in each treatment. *P<0.05, **P<0.005, ***P< vs the value before treatment (anova and paired t-test). British Journal of Anaesthesia  , DOI: ( /bja/aen265) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

4 Fig 3 Comparison of effects of various concentrations of clonidine and lidocaine on axonal transport. Values were obtained after a 1 h treatment with clonidine (a), lidocaine (b), or a combination of clonidine and lidocaine (c). Data are expressed as mean (sem) of five experiments in each treatment. *P<0.05, **P<0.005, ***P< vs corresponding direction in PSS treatment. Significance of the difference was examined by anova and Fisher's PLSD test. British Journal of Anaesthesia  , DOI: ( /bja/aen265) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

5 Fig 4 Changes in axonal transport of organelles before and during treatment with 10 μM TTX (a), 100 μM veratridine (b), a combination of 100 μM veratridine and 100 μM lidocaine (c), a combination of 1 μM ω-conotoxin MVIIA and 100 μM lidocaine (d), and a combination of 10 μM nifedipine and 100 μM lidocaine (e). Data are expressed as mean (sd) of five experiments in each treatment. *P<0.05, **P<0.005, ***P< vs the value before treatment (anova and paired t-test). British Journal of Anaesthesia  , DOI: ( /bja/aen265) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

6 Fig 5 Axonal transport after a 1 h treatment with PSS, 10 μM clonidine, 100 μM lidocaine, a combination of 10 μM clonidine and 100 μM lidocaine, a combination of 10 μM yohimbine, 10 μM clonidine, and 100 μM lidocaine, 10 μM TTX, 100 μM veratridine, a combination of 100 μM veratridine and 100 μM lidocaine, a combination of 1 μM ω-conotoxin MVIIA and 100 μM lidocaine, and a combination of 10 μM nifedipine and 100 μM lidocaine. Data are expressed as mean (sem) of five experiments in each treatment. ***P< vs corresponding direction in PSS treatment. #P<0.05, ##P<0.005, vs corresponding direction in veratridine alone treatment. Significance of the difference was examined by anova and Fisher's PLSD test. British Journal of Anaesthesia  , DOI: ( /bja/aen265) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

7 Fig 6 Neurite growth rate in cultured DRG neurones. Neurite growth was measured by video microscopy in DRG neurones during a 1 h treatment with PSS, 10 μM clonidine, 100 μM lidocaine, a combination of 10 μM clonidine and 100 μM lidocaine, a combination of 10 μM yohimbine, 10 μM clonidine, and 100 μM lidocaine, 10 μM TTX, 100 μM veratridine, a combination of 100 μM veratridine and 100 μM lidocaine, a combination of 1 μM ω-conotoxin MVIIA and 100 μM lidocaine, and a combination of 10 μM nifedipine and 100 μM lidocaine. Data are expressed as mean (sem). The numbers in the bars indicate the number of neurites examined. *P<0.05, **P<0.005 vs PSS treatment (anova and Fisher's PLSD test). British Journal of Anaesthesia  , DOI: ( /bja/aen265) Copyright © 2008 British Journal of Anaesthesia Terms and Conditions

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