1. A C 1 2 4 3 5 6
Segment 1
Segment 1 6 2 B
Segment 4 D 5 3
Peak diastolic strain rate
Peak systolic strain rate
Figure S1. Illustration of VVI measurements. Similar circumferential strain rate curves of a segment in left ventricular anterior wall were obtained in a normal dog (A) and a normal rat (B). Left ventricle was divided into six segments. In this study, we analyzed segment 1 and 4 to represent ischemic and non-ischemic region, respectively. Yellow and purple curves denote circumferential strain rate in ischemic and non-ischemic region, respectively. A cardiac cycle was marked by two vertical red lines. Circumferential velocity, strain and strain rate curves of a single speckle in left ventricular anterior wall in a normal dog (C) and a normal rat (D), similar curves were obtained between the dog and the rat.

2. A *
LV end-diastolic diameter (mm)
Reperfusion (min) B *
LV end-systolic diameter (mm)
Reperfusion (min)
Figure S2. Over time changes of left ventricular (LV) dimensions measured with echocardiography. (A) Illustration of LV end-diastolic diameter. (B) Illustration of LV end-systolic diameter. Pre: pre-ischemia; IS: ischemia; *p < vs. Pre (pre-occlusion) in the 4 ischemia groups, n = 5 in each group.

3. A
Left ventricular fractional shortening (%)
r = 0.647, p < 0.001
Maximal LVP rising rate (mmHg/s) B
Left ventricular fractional shortening (%)
r = 0.544, p = 0.002
Maximal LVP descending rate (mmHg/s)
Figure S3. Correlations between left ventricular fractional shortening (LVFS) measured by echocardiography and invasive hemodynamic parameters. (A) Correlation between LVFS and dp/dt max (maximal left ventricular pressure (LVP) rising rate). (B) Correlation between LVFS and dp/dt min (Maximal LVP descending rate). Data at time points of pre-ischemia, end of ischemia and 30 min after reperfusion in ischemia 5- and 15-min groups were used (n = 30).