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Follicle-stimulating hormone receptor polymorphism (Thr307Ala) is associated with variable ovarian response and ovarian hyperstimulation syndrome in Indian.

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Presentation on theme: "Follicle-stimulating hormone receptor polymorphism (Thr307Ala) is associated with variable ovarian response and ovarian hyperstimulation syndrome in Indian."— Presentation transcript:

1 Follicle-stimulating hormone receptor polymorphism (Thr307Ala) is associated with variable ovarian response and ovarian hyperstimulation syndrome in Indian women  Swati K. Achrekar, M.Sc., Deepak N. Modi, Ph.D., Sadhana K. Desai, M.D., Vijay S. Mangoli, Ph.D., Ranjana V. Mangoli, M.Sc., Smita D. Mahale, Ph.D.  Fertility and Sterility  Volume 91, Issue 2, Pages (February 2009) DOI: /j.fertnstert Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 (A) RFLP analysis of the Thr307Ala FSH receptor polymorphism. Lane 1: PCR product of external PCR using primer set as mentioned in Methodology (447 bp). Lane 2: PCR product using nested primers as in Reference 24 (364 bp). Lane 3: PhiX174 DNA Hinf I ladder. Lanes 4–10: digested PCR products showing the polymorphism. Lanes 4, 6, 7, and 9: two fragments, 364 bp and 328 bp, indicates heterozygous TA. Lanes 5 and 8: 364-bp fragment for homozygous T. Lane 10: 328-bp fragment for homozygous A. (B) Electropherogram showing the polymorphism at position 307. TT showing homozygous T allele (arrow), TA showing heterozygosity (arrow), and AA showing homozygous A allele (arrow). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 (A) RFLP analysis of the Asn680Ser FSH receptor polymorphism. Lane 1: phiX174 DNA Hinf I ladder. Lane 2: undigested PCR product of 520 bp. Lanes 3–7: digested PCR products showing the polymorphism. Lanes 3 and 5: two fragments of 413 bp and 107 bp for homozygous S. Lane 4: 520-bp fragment for homozygous N. Lanes 6 and 7: three fragments of 520 bp, 413 bp, and 107 bp for heterozygous NS. (B) Electropherogram showing the polymorphism at position 680. NN showing homozygous N allele (arrow), NS showing heterozygosity (arrow), and SS showing homozygous S allele (arrow). Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

4 Figure 3 Distribution of the FSH receptor genotypes at position 307 (A) and position 680 (B) in controls and subjects undergoing ART. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions


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