1. A Thermodynamic Study of the Effects of Cholesterol on the Interaction between Liposomes and Ethanol 
Christa Trandum, Peter Westh, Kent Jørgensen, Ole G. Mouritsen 
Biophysical Journal 
Volume 78, Issue 5, Pages (May 2000)
DOI: /S (00)
Copyright © 2000 The Biophysical Society Terms and Conditions

2. Figure 1
Schematic phase diagram for mixtures of DMPC and cholesterol (From Ipsen et al. 1987). Tm is the main transition temperature of pure DMPC. The different phases are labeled: so, solid-ordered, the gel phase; ld, liquid-disordered, the fluid phase; and lo, liquid-ordered.
Biophysical Journal  , DOI: ( /S (00) )
Copyright © 2000 The Biophysical Society Terms and Conditions

3. Figure 2
Measured values of the partial enthalpy, HlipidE, of DMPC liposomes of different cholesterol content in ethanol solutions calculated according to Eq. 1 and plotted as a function of the ethanol molality, methanol at 28°C. ○, 0mol%; ●, 0.5%; □, 1%; ■, 5%; △, 10%; ▴, 15%; ▿, 20%; ▾, 25%; ♢, 30%; and ♦, 40% mol% cholesterol.
Biophysical Journal  , DOI: ( /S (00) )
Copyright © 2000 The Biophysical Society Terms and Conditions

4. Figure 3
The interaction parameter, Hlipid–alcohol, as a function of cholesterol concentration at 28°C. The interaction parameter is taken directly as the slope of the curves in Fig. 2. The partitioning coefficient of ethanol (mole fraction scale) is plotted as a function of the cholesterol concentration in the inset.
Biophysical Journal  , DOI: ( /S (00) )
Copyright © 2000 The Biophysical Society Terms and Conditions

5. Figure 4
(A) The temperature dependence of the interaction parameter, Hlipid–alcohol. (B) The temperature dependence of (○) partitioning coefficients of ethanol into pure DMPC bilayers and (□) partitioning coefficients determined from the interaction parameter, Hlipid–alcohol. Applying a standard partitioning model, where ethanol is considered distributed between the membrane and the aqueous phase, partitioning coefficients (in molal units) can be estimated from the interaction parameters using the equation Km=Hlipid–ethanolE/ΔH°Mlipid (Trandum et al., 1999b).
Biophysical Journal  , DOI: ( /S (00) )
Copyright © 2000 The Biophysical Society Terms and Conditions

6. Figure 5
The temperature dependence of the interaction parameter, Hlipid–alcohol, at various cholesterol concentrations. ○, Pure DMPC; □, 4; △, 15; ▿, 25; and ♢, 35mol%.
Biophysical Journal  , DOI: ( /S (00) )
Copyright © 2000 The Biophysical Society Terms and Conditions