1. Ovarian tissue cryopreservation in female-to-male transgender people: insights into ovarian histology and physiology after prolonged androgen treatment 
Chloë De Roo, Sylvie Lierman, Kelly Tilleman, Karen Peynshaert, Kevin Braeckmans, Mirte Caanen, Cornelius B. Lambalk, Steven Weyers, Guy T'Sjoen, Ria Cornelissen, Petra De Sutter 
Reproductive BioMedicine Online 
Volume 34, Issue 6, Pages (June 2017)
DOI: /j.rbmo
Copyright © 2017 Reproductive Healthcare Ltd. Terms and Conditions

2. Figure 1
Follicle classification. Hematoxylin/eosin staining: (A) primordial follicle, 40x (scale bar = 20 µm); (B) intermediate follicle, 40x (scale bar = 20 µm); (C) primary follicle, 40x (scale bar = 20 µm); (D) secondary follicle, 40x (scale bar = 20 µm).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
(A) Cumulus oocytes complexes during manipulation of ovarian cortex tissue and before maturation (scale bar =  µm). After 48 h in-vitro maturation: (B) germinal vesicle oocyte (scale bar = 31.30 µm); (C) germinal vesicle breakdown oocyte or metaphase I oocyte (scale bar = 28.30 µm); (D) metaphase II oocyte (scale bar 31.80 µm).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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4. Figure 3
Confocal imaging section of meiotic spindle configuration of in-vitro matured MII oocytes. Chromosomes were stained red and microtubuli stained green. Normal spindle formation (Figure 3a and 3b); (A) barrel shaped microtubuli, which formed two opposite pointed poles (*), bipolar. Aligned chromosomes at the equatorial region (arrow); (B) slightly aberrant shaped microtubules with no pointed poles (**) and six chromosomes or less separated from the equatorial region (arrow). Abnormal spindle formation (Figure 3c and 3d); (C) irregularities of the microtubuli at the equatorial region and at the poles. Chromosomes away from equatorial region and located throughout the spindle (arrow); (D) barrelled shaped poles (**) with more than six chromosomes away from equatorial region (arrow).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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5. Figure 4
Correlation between anti-Müllerian serum levels and cumulus-oocyte complexes. Linear regression analysis y = 7.48x (b0 = 0.847; non-significant; 95% CI −10.44 to 12.19; b1 = 7.48; P < 0.001; 95% CI 5.73 to 9.23; R ). AMH, anti-Müllerian hormone.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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6. Figure 5
Hematoxylin/eosin staining of an entire ovary (scale bar 2000 µm). Antral follicles are located on the boundary between cortex (*) and medulla (**).
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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