1. Serum IgG subclass representation from BALB/c mice (n = 5/group) following COBRA P1 virus i.n., i.p., or i.m. prime followed by i.p. or i.m. boost.
Serum IgG subclass representation from BALB/c mice (n = 5/group) following COBRA P1 virus i.n., i.p., or i.m. prime followed by i.p. or i.m. boost. Titer was determined using day 35–59 pooled postboost sera and reported as absolute mean value plus SEM obtained in a single experiment and using sera from different mouse groups. Data are representative of two independent experiments evaluating the serum IgG subclass distribution of the same mouse groups against the COBRA P1 rHA Ag. For each independent experiment, mouse sera were assayed in duplicate. Micrograms of IgG1, IgG2a, and IgG2b standards per milliliter (micrograms per milliliter) equivalents were determined by interpolating the O.D.414 values obtained from the serum samples with those obtained from the corresponding IgG1, IgG2a and IgG2b standards. ***p < 0.001, **0.001 ≤ p < 0.05.
Giuseppe A. Sautto et al. ImmunoHorizons 2018;2:
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