1. Differential cardiac gene expression during cardiopulmonary bypass: Ischemia- independent upregulation of proinflammatory genes 
Mihai V. Podgoreanu, MD, Gregory A. Michelotti, PhD, Yukie Sato, MD, Michael P. Smith, MSc, Simon Lin, MD, Richard W. Morris, PhD, Hilary P. Grocott, MD, Joseph P. Mathew, MD, Debra A. Schwinn, MD 
The Journal of Thoracic and Cardiovascular Surgery 
Volume 130, Issue 2, Pages (August 2005)
DOI: /j.jtcvs
Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

2. Figure 1
Schematic diagram of the surgical preparation and rat CPB apparatus. (From Mackensen GB, Sato Y, Nellgard B, Pineda J, Newman MF, Warner DS, Grocott HP. Cardiopulmonary bypass induces neurologic and neurocognitive dysfunction in the rat. Anesthesiology. 2001;95:1485–91. Reproduced with permission of Lippincott Williams & Wilkins.)
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )
Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

3. Figure 2
Volcano plot of significance against effect. Bayesian statistical approaches were used to assess the statistical significance of microarray data, as described in the “Methods” section. For each gene, a Bayesian B score is calculated and plotted versus the average difference between the 2 experimental groups (M score). By using stringent statistical cutoff values, 25 downregulated (green) and 17 upregulated genes (red) were identified in response to CPB.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )
Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

4. Figure 3
Unsupervised hierarchical clustering of 42 myocardial genes differentially expressed with CPB. Data from animals undergoing CPB are presented in the left 3 columns, and data on sham control animals are presented in the right 3 columns. The upper panel demonstrates genes downregulated (green), whereas the lower panel shows genes upregulated (red) compared with sham control animals. For each gene, the mean normalized hybridization intensity is calculated within each experimental group, and relative expression of each animal is presented relative to the calculated mean. A list of the exact genes shown is further detailed in Table 2.
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )
Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions

5. Figure 4
Confirmation of microarray data by means of real-time quantitative RT-PCR in a subset of 7 genes differentially expressed with CPB. Primers for each gene are listed in the “Methods” section. GAPDH was used to normalize mRNA levels between samples, and values for each gene are shown as fold change in CPB-treated animals versus those seen in sham-operated control animals (mean ± SEM; results represent n = 3 for 3 independent experimental groups).
The Journal of Thoracic and Cardiovascular Surgery  , DOI: ( /j.jtcvs )
Copyright © 2005 The American Association for Thoracic Surgery Terms and Conditions