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Comprehensive Hybrid Capture–Based Next-Generation Sequencing Identifies a Double ALK Gene Fusion in a Patient Previously Identified to Be False-Negative.

Published byWidyawati Yulia Chandra Modified over 6 years ago

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Presentation on theme: "Comprehensive Hybrid Capture–Based Next-Generation Sequencing Identifies a Double ALK Gene Fusion in a Patient Previously Identified to Be False-Negative."— Presentation transcript:

1 Comprehensive Hybrid Capture–Based Next-Generation Sequencing Identifies a Double ALK Gene Fusion in a Patient Previously Identified to Be False-Negative by FISH  Johannes M. Heuckmann  Journal of Thoracic Oncology  Volume 12, Issue 3, Pages e22-e24 (March 2017) DOI: /j.jtho Copyright © 2016 International Association for the Study of Lung Cancer Terms and Conditions

2 Figure 1 (A) Anaplastic lymphoma kinase immunohistochemistry (clone 5A4, Novocastra [Leica Biosystems, Amsterdam, the Netherlands]) was performed on tissue showing a strong staining of the tumor cells. (B) Fluorescence in situ hybridization analysis (Vysis LSI ALK Dual Colour, Break Apart Rearrangement Probe [Vysis, Downers Grove, IL]) showed more than 95% fusion-negative tumor cells. Journal of Thoracic Oncology  , e22-e24DOI: ( /j.jtho ) Copyright © 2016 International Association for the Study of Lung Cancer Terms and Conditions

3 Figure 2 Schematic of the two anaplastic lymphoma receptor tyrosine kinase gene (ALK) rearrangements is shown. First, an echinoderm microtubule associated protein like 4 gene (EML4)-ALK gene fusion is formed, followed by a second double-strand break forming a WD repeat domain 43 gene (WDR43)-ALK gene fusion. Genomic distances are indicated at the top. The position of the 5′ fluorescence in situ hybridization probe is represented by the green bar and the 3′ probe by the red bar. Bold arrows indicate genomic rearrangements. Fine arrows indicate sense and antisense orientation of the respective gene. Journal of Thoracic Oncology  , e22-e24DOI: ( /j.jtho ) Copyright © 2016 International Association for the Study of Lung Cancer Terms and Conditions

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