Presentation is loading. Please wait.

Presentation is loading. Please wait.

Iontophoresis and Sonophoresis Stimulate Epidermal Cytokine Expression at Energies That Do Not Provoke a Barrier Abnormality: Lamellar Body Secretion.

Published byVũ Dương Modified over 5 years ago

Similar presentations

Presentation on theme: "Iontophoresis and Sonophoresis Stimulate Epidermal Cytokine Expression at Energies That Do Not Provoke a Barrier Abnormality: Lamellar Body Secretion."— Presentation transcript:

1 Iontophoresis and Sonophoresis Stimulate Epidermal Cytokine Expression at Energies That Do Not Provoke a Barrier Abnormality: Lamellar Body Secretion and Cytokine Expression Are Linked to Altered Epidermal Calcium Levels  Eung Ho Choi, Min Jung Kim, Sung Ku Ahn  Journal of Investigative Dermatology  Volume 121, Issue 5, Pages (November 2003) DOI: /j x Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

2 Figure 1 Nile red stain after US. Nile red stain was used to observe neutral lipids in SC. After US, the fluorescence in the SC was decreased with the immersion in PBS containing calcium compared to PBS only and in NC. US(PBS), immersion in PBS after ultrasound treatment; US(PBS+Ca2+), immersion in PBS containing calcium after ultrasound treatment; NC(PBS), immersion in PBS without treatment; NC(PBS+Ca2+), immersion in PBS containing calcium without treatment. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

3 Figure 2 Secretion of LB in the SC–SG junction after US was increased compared to NC. It was inhibited by immersion in PBS containing calcium compared to PBS only. US(PBS), immersion in PBS after ultrasound treatment; US(PBS+Ca2+), immersion in PBS containing calcium after ultrasound treatment; US(air), air exposure after ultrasound treatment. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

4 Figure 3 IL-1α, TNF-α, and TGF-β expression are increased in murine epidermis at 1 h after iontophoresis and ultrasound on skin surface. Total RNA was extracted, reverse-transcribed, and analyzed by real-time quantitative RT-PCR using GAPDH as an endogenous control. Results are illustrated relative to the mRNA levels of NC. All three cytokines showed increased expression in iontophoresis treated skin for 1 h (Ion 1 h) and 2 h (Ion 2 h) and the ultrasound-treated skin (US) compared to the NC as well as tape-stripped skin (TS) used as a positive control. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

5 Figure 4 IL-1α, TNF-α, and TGF-β expression are increased in murine epidermis at 2 h after iontophoresis and ultrasound on skin surface. Total RNA was extracted, reverse-transcribed, and analyzed by real-time quantitative RT-PCR using GAPDH as an endogenous control. Results are illustrated relative to the mRNA levels of the NC. All three cytokines showed increased expression in iontophoresis treated skin for 1 h (Ion 1 h) and 2 h (Ion 2 h) and ultrasound-treated skin (US) compared to the NC as well as tape-stripped skin (TS) used as a positive control. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

6 Figure 5 Immunohistochemical stain of TNF-α after 2 h positive iontophoresis.nl, control; 1h, 1 h after iontophoresis; 3h, 3 h after iontophoresis; 6h, 6 h after iontophoresis. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

7 Figure 6 IL-1α expression after iontophoresis.nl, control; 1h, 1 h after iontophoresis; 3h, 3 h after iontophoresis; 6h, 6 h after iontophoresis. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

8 Figure 7 TNF-α expression after iontophoresis.nl, control; 1h, 1 h after iontophoresis; 3h, 3 h after iontophoresis; 6h, 6 h after iontophoresis. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

9 Figure 8 IL-1α, TNF-α, and TGF-β expression are controlled by the calcium ion. (A) The expression of mRNA of each cytokines was decreased in murine epidermis by 2 h of immersion in PBS including calcium ion compared to PBS only (control) after iontophoresis (1 h) and ultrasound on skin surface. All three cytokines showed less expression by addition of the calcium ion. (B) The expression of mRNA of IL-1α and TNF-α was decreased in high-calcium iontophoresis compared to low calcium. Total RNA was extracted, reverse-transcribed, and analyzed by real-time quantitative RT-PCR using GAPDH as an endogenous control. Results are illustrated as the reduction rate (percentage) on the control. IPC, 2 h of immersion in PBS including calcium ion after 1 h iontophoresis; UPC, 2 h of immersion in PBS including calcium ion after ultrasound treatment. Journal of Investigative Dermatology  , DOI: ( /j x) Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

Download ppt "Iontophoresis and Sonophoresis Stimulate Epidermal Cytokine Expression at Energies That Do Not Provoke a Barrier Abnormality: Lamellar Body Secretion."

Similar presentations

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway  Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,
Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.

Loss of Extracellular Superoxide Dismutase Induces Severe IL-23-Mediated Skin Inflammation in Mice  Yun Sang Lee, In-Su Cheon, Byung-Hak Kim, Myung-Ja.
Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.

Differential Regulation by IL-1β and EGF of Expression of Three Different Hyaluronan Synthases in Oral Mucosal Epithelial Cells and Fibroblasts and Dermal.
Atopic March from Atopic Dermatitis to Asthma-Like Lesions in NC/Nga Mice Is Accelerated or Aggravated by Neutralization of Stratum Corneum but Partially.

Atopic March from Atopic Dermatitis to Asthma-Like Lesions in NC/Nga Mice Is Accelerated or Aggravated by Neutralization of Stratum Corneum but Partially.
Ultraviolet Radiation Increases Tropoelastin mRNA Expression in the Epidermis of Human Skin In Vivo  Jin Young Seo, Seong Hun Lee, Choon Shik Youn, Hai.

Ultraviolet Radiation Increases Tropoelastin mRNA Expression in the Epidermis of Human Skin In Vivo  Jin Young Seo, Seong Hun Lee, Choon Shik Youn, Hai.
Negative Electric Potential Induces Alteration of Ion Gradient and Lamellar Body Secretion in the Epidermis, and Accelerates Skin Barrier Recovery After.

Negative Electric Potential Induces Alteration of Ion Gradient and Lamellar Body Secretion in the Epidermis, and Accelerates Skin Barrier Recovery After.
The Protective Effects of Melittin on Propionibacterium acnes–Induced Inflammatory Responses In Vitro and In Vivo  Woo-Ram Lee, Kyung-Hyun Kim, Hyun-Jin.

The Protective Effects of Melittin on Propionibacterium acnes–Induced Inflammatory Responses In Vitro and In Vivo  Woo-Ram Lee, Kyung-Hyun Kim, Hyun-Jin.
Topical Application of 17β-Estradiol Increases Extracellular Matrix Protein Synthesis by Stimulating TGF-β Signaling in Aged Human Skin In Vivo  Eui Dong.

Topical Application of 17β-Estradiol Increases Extracellular Matrix Protein Synthesis by Stimulating TGF-β Signaling in Aged Human Skin In Vivo  Eui Dong.
A Toll-Like Receptor 7, 8, and 9 Antagonist Inhibits Th1 and Th17 Responses and Inflammasome Activation in a Model of IL-23-Induced Psoriasis  Weiwen.

A Toll-Like Receptor 7, 8, and 9 Antagonist Inhibits Th1 and Th17 Responses and Inflammasome Activation in a Model of IL-23-Induced Psoriasis  Weiwen.
Expression of Protease-Activated Receptor-2 in SZ95 Sebocytes and its Role in Sebaceous Lipogenesis, Inflammation, and Innate Immunity  Sang E. Lee, Ji-Min.

Expression of Protease-Activated Receptor-2 in SZ95 Sebocytes and its Role in Sebaceous Lipogenesis, Inflammation, and Innate Immunity  Sang E. Lee, Ji-Min.
Insulin-Like Growth Factor-I Enhances Transforming Growth Factor-β-Induced Extracellular Matrix Protein Production Through the P38/Activating Transcription.

Insulin-Like Growth Factor-I Enhances Transforming Growth Factor-β-Induced Extracellular Matrix Protein Production Through the P38/Activating Transcription.
IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft- Versus-Host Disease  Doanh Le Huu, Takashi Matsushita, Guihua Jin, Yasuhito.

IL-6 Blockade Attenuates the Development of Murine Sclerodermatous Chronic Graft- Versus-Host Disease  Doanh Le Huu, Takashi Matsushita, Guihua Jin, Yasuhito.
Peter M. Elias, Sung K. Ahn, Mitsuhiro Denda, Barbara E

Peter M. Elias, Sung K. Ahn, Mitsuhiro Denda, Barbara E
HCR, a Candidate Gene for Psoriasis, Is Expressed Differently in Psoriasis and Other Hyperproliferative Skin Disorders and Is Downregulated by Interferon-γ.

HCR, a Candidate Gene for Psoriasis, Is Expressed Differently in Psoriasis and Other Hyperproliferative Skin Disorders and Is Downregulated by Interferon-γ.
Penetration Pathways Induced by Low-Frequency Sonophoresis with Physical and Chemical Enhancers: Iron Oxide Nanoparticles versus Lanthanum Nitrates  Sang.

Penetration Pathways Induced by Low-Frequency Sonophoresis with Physical and Chemical Enhancers: Iron Oxide Nanoparticles versus Lanthanum Nitrates  Sang.
25 Hydroxyvitamin D 1 α-Hydroxylase Is Required for Optimal Epidermal Differentiation and Permeability Barrier Homeostasis  D.D. Bikle, S. Chang, D. Crumrine,

25 Hydroxyvitamin D 1 α-Hydroxylase Is Required for Optimal Epidermal Differentiation and Permeability Barrier Homeostasis  D.D. Bikle, S. Chang, D. Crumrine,
Shao Jun Jiang, Sang Min Hwang, Eung Ho Choi, Sung Ku Ahn 

Shao Jun Jiang, Sang Min Hwang, Eung Ho Choi, Sung Ku Ahn 
Rottlerin, a Specific Inhibitor of Protein Kinase C-delta, Impedes Barrier Repair Response by Increasing Intracellular Free Calcium  Bong K. Ahn, Se K.

Rottlerin, a Specific Inhibitor of Protein Kinase C-delta, Impedes Barrier Repair Response by Increasing Intracellular Free Calcium  Bong K. Ahn, Se K.
Enhancing 1α-Hydroxylase Activity with the 25-Hydroxyvitamin D-1α-Hydroxylase Gene in Cultured Human Keratinocytes and Mouse Skin  Tai C. Chen, Xue Hong.

Enhancing 1α-Hydroxylase Activity with the 25-Hydroxyvitamin D-1α-Hydroxylase Gene in Cultured Human Keratinocytes and Mouse Skin  Tai C. Chen, Xue Hong.
Regulation of IL-33 Expression by IFN-γ and Tumor Necrosis Factor-α in Normal Human Epidermal Keratinocytes  Jitlada Meephansan, Hidetoshi Tsuda, Mayumi.

Regulation of IL-33 Expression by IFN-γ and Tumor Necrosis Factor-α in Normal Human Epidermal Keratinocytes  Jitlada Meephansan, Hidetoshi Tsuda, Mayumi.

Similar presentations

Ads by Google