1. Hippocampus and Entorhinal Cortex Recruit Cholinergic and NMDA Receptors Separately to Generate Hippocampal Theta Oscillations 
Zhenglin Gu, Georgia M. Alexander, Serena M. Dudek, Jerrel L. Yakel 
Cell Reports 
Volume 21, Issue 12, Pages (December 2017)
DOI: /j.celrep
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2. Cell Reports 2017 21, 3585-3595DOI: (10.1016/j.celrep.2017.11.080)
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3. Figure 1
Cholinergic Receptors in the Hippocampus Are Critical for Hippocampal Theta Generation
(A) Representative hippocampal local field potential recordings from one mouse freely moving in an open field arena over four trials under various intra-hippocampal antagonist infusion treatments.
(B) Spectrogram analysis of representative in vivo hippocampal field potential recordings (20 s) showing strong theta power during running. Theta power was impaired by hippocampal infusion of either the AMPA receptor antagonist CNQX or the muscarinic blocker atropine, but not by the NMDA receptor antagonist APV.
(C) Power spectral density analysis of representative recordings showing the power profile after various treatments.
(D) Bar graph showing normalized peak theta power after infusion of different receptor antagonists to the ipsilateral hippocampus. ∗∗∗p = (CNQX), ∗∗∗p = (atropine), and p = (APV) compared with saline treatment, n = 6 mice per treatment group, one-way ANOVA, Holm-Sidak post hoc test.
(E) Bar graph showing that peak theta frequency was not significantly changed by any antagonist treatments. p = between groups, n = 6 mice per group, one-way ANOVA.
(F) Bar graph showing that Y-maze spontaneous alternation task performance was impaired by hippocampal infusion of either CNQX or atropine, but not by APV. ∗p = for CNQX and ∗p =  for atropine compared with saline treatment, n = 7 mice per treatment group, one-way ANOVA, Holm-Sidak post hoc test.
(G) Bar graph showing that the total number of Y-maze arm entries was not significantly changed over the four treatments. p = between treatment groups, n = 7 mice per group, one-way ANOVA.
Error bar depicts SEM.
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4. Figure 2
NDMA Receptors in the EC Are Critical for Hippocampal Theta Generation
(A) Representative hippocampal local field potential recordings from one mouse freely moving in an open field arena over four trials under various intra-EC antagonist infusion treatments.
(B) Spectrogram analysis of representative in vivo hippocampal field potential recordings (20 s) showing that the theta power during running was impaired by EC infusion of either the AMPA receptor antagonist CNQX or by the NMDA receptor antagonist APV, but not by the muscarinic blocker atropine.
(C) Power spectral density analysis of representative recordings showing the power profile after various treatments.
(D) Bar graph showing normalized peak theta power after infusion of different receptor antagonists to ipsilateral EC. ∗∗∗p = (CNQX), ∗∗∗p =  (APV), and p = (atropine) compared with saline treatment, n = 6 mice per treatment group, one-way ANOVA, Holm-Sidak post hoc test.
(E) Bar graph showing that theta frequency was not significantly changed by any antagonist treatments. p = between groups, n = 6 mice per group, one-way ANOVA.
(F) Bar graph showing that Y-maze spontaneous alternation task was impaired by EC infusion of either CNQX or APV, but not by atropine. ∗∗∗p =  for CNQX, ∗∗∗p = for APV, and p =  for atropine compared with saline treatment, n = 7 mice per treatment group, one-way ANOVA, Holm-Sidak post hoc test.
(G) Bar graph showing that the total number of Y-maze arm entries was not significantly changed over the four treatments. p = between treatments, n = 7 mice per group, one-way ANOVA.
Error bar depicts SEM.
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5. Figure 3
Cholinergic Receptor Activation Is Required for the Induction of Theta Oscillation during the Pairing, but Not for the Expression of Theta after the Pairing, in Slice Co-culture Preparation
(A) The scheme of in vitro induction of theta-like oscillations in slice co-cultures. Field potentials were recorded from CA1. The Schaffer collateral (SC) pathway was activated by a stimulating electrode. Cholinergic neurons were activated via channelrhodopsin-2 (ChR2) that was specifically expressed in ChAT-positive neurons.
(B) Induction of theta-like oscillations by paring SC and cholinergic activation, but not by either pathway activation alone. After 5–10 pairings, SC stimulation alone could then induce theta oscillations.
(C) Power spectral density analysis of representative traces before, during, and 30 min after the pairing showing the peak power around 8.5 Hz.
(D) Bar graphs showing similar peak power spectral density (43.2 ± 6.2 μV2/Hz at 30 min after pairing versus 47.0 ± 7.6 μV2/Hz during pairing, n = 6 slices for each group, p = 0.70, t test) and frequency (8.4 ± 0.35 Hz at 30 min after pairing versus 8.6 ± 0.41 Hz during pairing, n = 6 slices for each group, p = 0.71, t test) during pairing and 30 min after the pairing.
(E) Representative traces showing that theta induction was blocked by bath-applied atropine.
(F) Bar graph of the peak power spectral density showing that the theta induction was blocked by either atropine or APV. ∗∗∗∗p < compared with control group, n = 6 slices per group, one-way ANOVA, Holm-Sidak post hoc test.
(G and H) Representative traces (G) and bar graph (H) showing that the theta expression was blocked by APV, but not by atropine. ∗∗∗∗p < compared with control, n = 5 slices for each treatment, n = 8 slices for control groups, one-way ANOVA, Holm-Sidak post hoc test.
Error bar depicts SEM.
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6. Figure 4
Cholinergic Inputs Target the Hippocampus to Induce Theta Oscillations In Vitro
(A and B) Representative traces (A) and bar graph (B) showing perfusion of atropine to CA1, but not EC before pairing blocked the induction of theta oscillations.
(C and D) Representative traces (C) and bar graph (D) showing perfusion of ACh to CA1, but not EC-induced theta oscillations when paired with SC stimulation. ∗∗∗∗p < compared with control, n = 5 slices for each group, one-way ANOVA, Holm-Sidak post hoc test.
Error bar depicts SEM.
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7. Figure 5
Cholinergic Tone Increases Excitatory Transmission and Decreases Inhibitory Transmission in the Hippocampus and Subsequent Outputs to the EC in Slice Co-cultures
(A) Normalized SC-evoked, whole-cell EPSP responses from CA1 pyramidal neurons showing that cholinergic pairing (at the time of 0 min as indicated by the arrow) increased SC-evoked EPSP amplitude, and the effect lasted at least 30 min after the pairing.
(B) Normalized SC-evoked EPSC responses from CA1 pyramidal neurons showing that cholinergic pairing persistently increased EPSC amplitude.
(C) Normalized SC-evoked IPSC responses from CA1 pyramidal neurons showing that cholinergic pairing persistently decreased IPSC amplitude.
(D) Bar graph showing that cholinergic pairing significantly increased SC to CA1 synaptic EPSP amplitude (∗∗p = 0.004, n = 6 slices, t test) and EPSC (∗∗p = 0.008, n = 6 slices, t test), whereas it decreased IPSC amplitude (∗∗∗p < 0.001, n = 6 slices, t test).
(E and F) Paired-pulse (100-ms interval) responses showing that cholinergic pairing did not significantly change the paired-pulse ratios for SC-evoked EPSP (p = 0.79, n = 7 slices, paired t test) (E) or IPSC responses (p = 0.83, n = 8 slices, paired t test) (F) in CA1.
(G–J) Similar plots for EC V pyramidal neurons as in (A)–(D) for CA1 pyramidal neurons. (G) Normalized SC-evoked EPSP responses from EC V pyramidal neurons showing that cholinergic pairing persistently increased EPSP amplitude. (H) Normalized SC-evoked EPSC responses from EC V pyramidal neurons showing that cholinergic pairing persistently increased EPSC amplitude. (I) Normalized SC-evoked IPSC responses from EC V pyramidal neurons showing that cholinergic pairing persistently decreased IPSC amplitude. (J) Bar graph showing that cholinergic pairing increased SC-stimulation-evoked EPSP (∗∗p = 0.002, n = 6 slices, t test) and EPSC (∗∗∗p < 0.001, n = 6 slices, t test) amplitudes, whereas it decreased IPSC (∗∗p = 0.004, n = 6 slices, t test) amplitudes recorded in EC V/VI pyramidal neurons.
(K and L) Paired-pulse (100-ms interval) responses showing that cholinergic pairing significantly decreased EPSC paired-pulse ratios (K) (∗∗p = 0.003, n = 6 slices, paired t test), whereas it increased IPSC ratios (L) in EC V/VI pyramidal neurons (∗∗p = 0.008, n = 7 slices, paired t test).
Error bar depicts SEM.
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8. Figure 6
Muscarinic M1 Receptors Mediate the Enhancement of Hippocampal Excitatory Transmission, but M4 Receptors Mediate the Depression of Hippocampal Inhibitory Transmission
(A) Normalized SC-evoked EPSC responses from CA1 pyramidal neurons showing that the enhancement of hippocampal excitatory transmission by cholinergic pairing (at the time of 0 min as indicated by the arrow) can be blocked by either the non-selective muscarinic receptor antagonist atropine or by the selective M1 receptor antagonist VU , but not by any of the other receptor subtype selective antagonists.
(B) Bar graph showing that only the non-selective muscarinic receptor antagonist atropine and the M1 receptor selective antagonist VU significantly blocked cholinergic enhancement of hippocampal excitatory transmission; antagonists selective for M2, M3, M4 and M5 did not block cholinergic enhancement of EPSC amplitude. ∗∗p =  for atropine and for VU compared with control, n = 6 slices per treatment group, one-way ANOVA, Holm-Sidak post hoc test.
(C) Normalized SC-evoked IPSC amplitude from CA1 pyramidal neurons showing that cholinergic depression of hippocampal inhibitory transmission can be blocked by either the non-specific muscarinic receptor antagonist atropine or by M4 receptor selective antagonist PD102807, but not by any of the other selective antagonists.
(D) Bar graph showing that, besides the non-specific muscarinic receptor antagonist (∗p = ), the M4 receptor selective antagonist PD (∗p = ) was the only one that significantly blocked cholinergic depression of hippocampal inhibitory transmission. Asterisks represent a comparison with control group, n = 6 slices per treatment group, one-way ANOVA, Holm-Sidak post hoc test.
(E and F) Normalized SC-evoked EPSC responses from CA1 pyramidal neurons (E) and bar graph (F) showing that cholinergic enhancement of hippocampal excitatory transmission can be blocked by knocking out M1 receptors in pyramidal neurons (CaMK2a-M1KO), but not in interneurons (GAD2-M1KO). ∗∗∗∗p < compared with control slices from floxed M1 mice, n = 6 slices per group, one-way ANOVA, Holm-Sidak post hoc test.
(G and H) Representative traces (G) and bar graph (H) showing that in vitro theta generation was impaired in slices with M1 receptor knockout in pyramidal neurons, but not in slices with M1 receptor knockout in interneurons. ∗∗∗∗p < compared with slices from floxed M1 controls, n = 6 slices per group, one-way ANOVA, Holm-Sidak post hoc test.
Error bar depicts SEM.
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9. Figure 7
Muscarinic M1 Receptors on the Pyramidal Neurons, but Not on the Inhibitory Neurons, Are Critical for Theta Generation and Y-Maze Spontaneous Alternation Performance
(A) Representative hippocampal local field potential recordings from mice with M1 mAChR knocked out from interneurons (GAD-M1) or pyramidal neurons (CaM-M1) or Floxed M1 control mice.
(B) Power spectral density analysis of representative recordings showing that the theta power was decreased in mice with M1 receptor knockout in pyramidal neurons, but not M1 receptor knockout in interneurons.
(C) Bar graph showing reduced peak theta power from mice with M1 knockout in pyramidal neurons (∗p = 0.032), but not M1 knockout in interneurons (p = 0.793). Asterisk represents a comparison with floxed M1 controls, n = 7 mice per group, one-way ANOVA, Holm-Sidak post hoc test.
(D) Bar graph showing that theta frequency was not significantly changed by M1 deletion. p = between groups, n = 7 mice per group, one-way ANOVA.
(E) Bar graph showing that Y-maze spontaneous alternation task performance was impaired in mice with the M1 receptor knockout in pyramidal neurons (∗p = 0.023), but not M1 receptor knockout in interneurons (p = 0.499). Asterisk represents a comparison with floxed M1 controls, n = 10 mice per group, one-way ANOVA, Holm-Sidak post hoc test.
(F) Bar graph showing that the total number of Y-maze arm entries was not significantly changed among the three groups (p = , n = 10 mice per group, one-way ANOVA).
Error bar depicts SEM.
Cell Reports  , DOI: ( /j.celrep )
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