1. Effect of VEGFA on ALDH+ population maintenance
Effect of VEGFA on ALDH+ population maintenance ALDH1‐positive and ALDH1‐negative cells were flow sorted. Western blots show Bmi1, pSrc, Src, and DNMT3A levels in sorted ALDH1+ and ALDH1− PEO1R cells.miR‐128 expression in ALDH1+ and ALDH1− PEO1R cells. *P =  Sorted ALDH1+ and ALDH1− PEO1R cells were plated into sphere assays ± VEGFA. Spheres > 75 μm were counted at 14 days. *P = 0.001, **P = 0.057.Sorted ALDH1+ and ALDH1− cells were reseeded into culture. The proportion of ALDH1− or ALDH1+ cells arising from ALDH1+ cells over the next 8 days in culture was serially assayed and graphed as mean % ± SEM.Growth curves of ALDH1+ progeny arising from ALDH1+ cells over 8 days in culture with or without added VEGFA.Data information: All graphed data show mean ± SEM for at least 3 different biologic experiments with at least three technical repeats within each assay. Differences between two groups were assayed by Student's t‐test. See also Fig EV6.
Kibeom Jang et al. EMBO Mol Med. 2017;emmm
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