Presentation is loading. Please wait.

Presentation is loading. Please wait.

Dendritic cells modulated by cytokine-expressing adenoviruses alleviate eosinophilia and airway hyperresponsiveness in an animal model of asthma  Yi-Ling.

Published byĐào Thắgn Modified over 5 years ago

Similar presentations

Presentation on theme: "Dendritic cells modulated by cytokine-expressing adenoviruses alleviate eosinophilia and airway hyperresponsiveness in an animal model of asthma  Yi-Ling."— Presentation transcript:

1 Dendritic cells modulated by cytokine-expressing adenoviruses alleviate eosinophilia and airway hyperresponsiveness in an animal model of asthma  Yi-Ling Ye, PhD, Yueh-Lun Lee, PhD, Zen-Jai Chuang, BS, Huai-Jean Lai, MS, Chun-Chi Chen, MS, Mi-Hua Tao, PhD, Bor-Luen Chiang, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 114, Issue 1, Pages (July 2004) DOI: /j.jaci

2 Fig 1 The expression efficiency of adenovirus-infected DCs. The bold area represents the GFP expression after Ad-GL-1 infection, and the filled line represents DCs without Ad-GL-1 infection (A). Different MOI of Ad-IL-12 (B) or Ad-IL-18 (C) or 3000 MOI of Ad-IL-12 combined with different MOI of Ad-IL-18 infected DCs (D, E) were analyzed by ELISA assay. Data represent the means ± SEMs. Journal of Allergy and Clinical Immunology  , 88-96DOI: ( /j.jaci )

3 Fig 2 The cytokine levels of CD4+ positive splenocytes from cytokine gene–modulated DC treated mice. CD4+ positive cells were co-cultured with OVA and irradiated splenocytes for 48 h in 96-well culture plates and then the cultured supernatant was collected for IL-4 (A), IL-5 secretion (B), or IFN-γ (C) cytokine assay. ∗P<.05; ∗∗P<.001; each adenovirus infected group vs DCs pulsed with OVA group. Journal of Allergy and Clinical Immunology  , 88-96DOI: ( /j.jaci )

4 Fig 3 Anti-OVA antibody expression levels of DC-treated mice. The IgG2a and IgE anti-OVA antibody expression from mice that received DCs pulsed with OVA (A) or without OVA (B) was measured by ELISA and shown as pg/mL. ∗P<.05 compared with the DC pulsed OVA group; ##P<.01 compared with the Ad-IL-12 infected group. Journal of Allergy and Clinical Immunology  , 88-96DOI: ( /j.jaci )

5 Fig 4 Preventive effect of modified DCs on AHR. The value of AHR (A) was measured after the challenge, and then BAL fluid samples were collected. Cells were counted and classified as monocytes (Mon), lymphocytes (Lym), neutrophils (Neu), or eosinophils (Eos)(B). Data represent the means ± SEMs of 7-8 mice per group. ∗∗P<.01, compared with DCs pulsed with OVA group. Journal of Allergy and Clinical Immunology  , 88-96DOI: ( /j.jaci )

6 Fig 5 Inflammatory mediator levels were analyzed in BAL fluid. BAL fluid samples were collected after the challenge. Eotaxin (A), KC (B), and IL-13 (C) levels in BAL fluid from mice were measured by ELISA assay. Data represent the means ± SEMs of 5-7 mice per group. ∗P<.05, compared with DC+OVA+Ad-mock group; #P<.05, compared with DC+OVA+Ad-IL-12 group. Journal of Allergy and Clinical Immunology  , 88-96DOI: ( /j.jaci )

7 Fig 6 Histopathologic study of lung tissue in mice that received the cytokine-modulated DCs. Histopathologic changes were examined by light microscopy for DC pulsed with OVA group mice (DC+OVA), Ad-Mock (DC+OVA+Ad-mock), Ad-IL-18 (DC+OVA+Ad-18), Ad-IL-12 (DC+OVA+Ad-12), and co-infected (DC+OVA+Ad-12+Ad-18) treated mice. Data showed different extent of cellular infiltration of the periairway region. Original magnification, ×200. Journal of Allergy and Clinical Immunology  , 88-96DOI: ( /j.jaci )

Download ppt "Dendritic cells modulated by cytokine-expressing adenoviruses alleviate eosinophilia and airway hyperresponsiveness in an animal model of asthma  Yi-Ling."

Similar presentations

Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression  Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,

Dual effects of vitamin D–induced alteration of TH1/TH2 cytokine expression  Victor Matheu, MD, Ove Bäck, MD, PhD, Emma Mondoc, BSc, Shohreh Issazadeh-Navikas,
Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein  Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.

Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein  Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.
Doxycycline reduces airway inflammation and hyperresponsiveness in a murine model of toluene diisocyanate–induced asthma  Kyung S. Lee, MS, Sun M. Jin,

Doxycycline reduces airway inflammation and hyperresponsiveness in a murine model of toluene diisocyanate–induced asthma  Kyung S. Lee, MS, Sun M. Jin,
CD4 T-helper cells engineered to produce IL-10 prevent allergen-induced airway hyperreactivity and inflammation  Jae-Won Oh, MD, PhD, Christine M. Seroogy,

CD4 T-helper cells engineered to produce IL-10 prevent allergen-induced airway hyperreactivity and inflammation  Jae-Won Oh, MD, PhD, Christine M. Seroogy,
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.

The activating protein 1 transcription factor basic leucine zipper transcription factor, ATF- like (BATF), regulates lymphocyte- and mast cell–driven immune.
The role of viruses in acute exacerbations of asthma

The role of viruses in acute exacerbations of asthma
Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,

Virus-specific IgE enhances airway responsiveness on reinfection with respiratory syncytial virus in newborn mice  Azzeddine Dakhama, PhD, Young-Mok Lee,
Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.

Allergy prevention starts before conception: Maternofetal transfer of tolerance protects against the development of asthma  Tobias Polte, PhD, Christian.
Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,

Interferon response factor 3 is essential for house dust mite–induced airway allergy  Thomas Marichal, DVM, Denis Bedoret, DVM, PhD, Claire Mesnil, DVM,
Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,

Innate IL-13–producing nuocytes arise during allergic lung inflammation and contribute to airways hyperreactivity  Jillian L. Barlow, PhD, Agustin Bellosi,
Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.

Expression of IL-4 receptor α on smooth muscle cells is not necessary for development of experimental allergic asthma  Frank Kirstein, PhD, William G.C.
Frank Kirstein, PhD, Natalie E

Frank Kirstein, PhD, Natalie E
Maternal house dust mite exposure during pregnancy enhances severity of house dust mite–induced asthma in murine offspring  Phoebe K. Richgels, MS, Amnah.

Maternal house dust mite exposure during pregnancy enhances severity of house dust mite–induced asthma in murine offspring  Phoebe K. Richgels, MS, Amnah.
Phosphodiesterase 4B is essential for TH2-cell function and development of airway hyperresponsiveness in allergic asthma  S.-L. Catherine Jin, PhD, Sho.

Phosphodiesterase 4B is essential for TH2-cell function and development of airway hyperresponsiveness in allergic asthma  S.-L. Catherine Jin, PhD, Sho.
IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,

IL-33 dysregulates regulatory T cells and impairs established immunologic tolerance in the lungs  Chien-Chang Chen, PhD, Takao Kobayashi, PhD, Koji Iijima,
Restoration of T-box–containing protein expressed in T cells protects against allergen- induced asthma  Jung Won Park, MD, Hyun Jung Min, MS, Jung Ho Sohn,

Restoration of T-box–containing protein expressed in T cells protects against allergen- induced asthma  Jung Won Park, MD, Hyun Jung Min, MS, Jung Ho Sohn,
Antigen-specific effector CD8 T cells regulate allergic responses via IFN-γ and dendritic cell function  Yafang Tang, BSc, Shou Ping Guan, BSc, Benson.

Antigen-specific effector CD8 T cells regulate allergic responses via IFN-γ and dendritic cell function  Yafang Tang, BSc, Shou Ping Guan, BSc, Benson.
Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation  Si-Yen Liu, PhD, Wei-Zhi Wang,

Leukocyte nicotinamide adenine dinucleotide phosphate-reduced oxidase is required for isocyanate-induced lung inflammation  Si-Yen Liu, PhD, Wei-Zhi Wang,
CD1d restricted natural killer T cells are not required for allergic skin inflammation  Abdallah Elkhal, PhD, Muriel Pichavant, PhD, Rui He, PhD, Jordan.

CD1d restricted natural killer T cells are not required for allergic skin inflammation  Abdallah Elkhal, PhD, Muriel Pichavant, PhD, Rui He, PhD, Jordan.

Similar presentations

Ads by Google