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Anaphylactic reaction to (mixed) fruit juice containing dragon fruit

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1 Anaphylactic reaction to (mixed) fruit juice containing dragon fruit
Andreas Kleinheinz, MD, Ute Lepp, MD, Björn M. Hausen, MD, Arnd Petersen, PhD, Wolf-Meinhard Becker, PhD  Journal of Allergy and Clinical Immunology  Volume 124, Issue 4, Pages (October 2009) DOI: /j.jaci Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Western blot of pitaya juice ([mixed] fruit juice containing dragon fruit) under nonreducing conditions, 15% SDS-PAGE: 1, protein staining (India Ink); 2, IgE reactivity of the patient's serum; 3, buffer control. The arrow indicates the position of the protein band excised for protein sequencing. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 Two-dimensional electrophoresis/immunoblotting of pitaya fruit extract: first dimension, pH 3 to 10; second dimension, 15% SDS-PAGE. A, Protein staining (Coomassie blue) of the 2-dimensional electrophoresis. B, Immunoblot depicting the IgE reactivity of the patient's serum. The marked spot was excised and N-terminally sequenced. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Western blot inhibition test. A, Western blot of pitaya fruit extract under nonreducing conditions, 15% SDS-PAGE: inhibition experiment. 1, protein staining (India Ink); 2–4, IgE reactivity of the patient's serum without inhibitor (2), with pitaya fruit extract as inhibitor (3), and with rTri a 14 (wheat LTP) as inhibitor (4). B, Western blot of peach extract containing Pru p 3 (LTP) under nonreducing conditions, 15% SDS-PAGE: 1, protein staining (India Ink); 2, rabbit anti–Cor a 8–reactive serum (cross-reacting with Pru p 3); 3 and 4, Pru p 3–positive sera; 5, serum of the pitaya-reactive patient; 6, anti–Cor a 8 control; 7, anti-IgE control. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci ) Copyright © 2009 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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