1. Expression of bnl in mesodermal bridge‐cells.
Expression of bnl in mesodermal bridge‐cells. (A,A′,B,B′) Whole‐mount antibody staining with anti‐GFP (green fluorescent protein; red) of stage 12–13 embryos bearing btl‐Gal4 and UAS–actin–GFP constructs. (A,A′) Hunchback (Hb; green) is expressed in the nucleus of the bridge‐cell (white arrowhead). (B,B′) Fluorescent in situ hybridization against bnl RNA (green). The spot necessary for DT elongation is indicated (white arrowhead). (C) Enzymatic co‐staining of Hb (immunostaining in brown) and bnl (in situ hybridization in blue) shows that bnl messenger RNA is produced by the bridge‐cell (black arrowhead). (D) Fluorescent triple staining of btl‐Gal4/UAS–actin–GFP embryos with anti‐GFP (blue), anti‐Hb (red) and bnl mRNA (green). (D′) Highlight of the region boxed in (D). The nucleus of the bridge‐cell (white arrowhead) is surrounded by the cytoplasmic bnl mRNA. WT, wild type.
Samir Merabet et al. EMBO Rep. 2005;6:
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