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Mrinal K. Sarkar, Parames C. Sil  Pathophysiology 

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Presentation on theme: "Mrinal K. Sarkar, Parames C. Sil  Pathophysiology "— Presentation transcript:

1 Hepatocytes are protected by herb Phyllanthus niruri protein isolate against thioacetamide toxicity 
Mrinal K. Sarkar, Parames C. Sil  Pathophysiology  Volume 14, Issue 2, Pages (October 2007) DOI: /j.pathophys Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 (a). Effect of the protein isolate on cell viability in isolated hepatocytes treated with TAA as well as (b) on ALT and (c) LDH leakages. Results have been given as percentage of control. Control: hepatocytes were treated with DMEM, TAA: hepatocytes exposed to TAA (2mM) for 60min. P1+TAA, P2+TAA, P3+TAA and P4+TAA: hepatocytes incubated with protein isolate (10, 25, 50 and 100μg/ml) prior to TAA administration for 60min, vitamin C+TAA: hepatocytes first incubated with vitamin C and then with TAA. Each value represents mean±S.D. (Pa<0.05; Pb<0.05). Pathophysiology  , DOI: ( /j.pathophys ) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

3 Fig. 2 Effect of the protein isolate on MDA levels in hepatocytes treated with TAA. Results have been given as percentage over control. Control: hepatocytes treated with DMEM, TAA: hepatocytes exposed to TAA (2mM) for 60min. P1+TAA, P2+TAA, P3+TAA and P4+TAA: hepatocytes incubated with protein isolate (10, 25, 50 and 100μg/ml) prior to TAA administration for 60min, vitamin C+TAA: hepatocytes first incubated with vitamin C and then with TAA. Each value represents mean±S.D. (Pa<0.05; Pb<0.05). Pathophysiology  , DOI: ( /j.pathophys ) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

4 Fig. 3 Effect of the protein isolate on GSH levels in hepatocytes. Results have been given as percentage of control. Control: hepatocytes treated with DMEM, TAA: hepatocytes exposed to TAA (2mM) for 60min. P1+TAA, P2+TAA, P3+TAA and P4+TAA: hepatocytes incubated with protein isolate (10, 25, 50 and 100μg/ml) prior to TAA administration for 60min, vitamin C+TAA: hepatocytes first incubated with vitamin C and then with TAA. Each value represents mean±S.D. (Pa<0.05; Pb<0.05). Pathophysiology  , DOI: ( /j.pathophys ) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

5 Fig. 4 (a). Effect of the protein isolate on catalase, (b) SOD and (c) GST activity in hepatocytes. Results have been given as percentage of control. Control: hepatocytes treated with DMEM, TAA: hepatocytes exposed to TAA (2mM) for 60min. P1+TAA, P2+TAA, P3+TAA and P4+TAA: hepatocytes incubated with protein isolate (10, 25, 50 and 100μg/ml) prior to TAA administration for 60min, vitamin C+TAA: hepatocytes first incubated with vitamin C and then with TAA. Each value represents mean±S.D. (Pa<0.05; Pb<0.05). Pathophysiology  , DOI: ( /j.pathophys ) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions

6 Fig. 5 DPPH scavenging activity of the protein isolate of Phyllanthus niruri with increasing concentrations. Pathophysiology  , DOI: ( /j.pathophys ) Copyright © 2007 Elsevier Ireland Ltd Terms and Conditions


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