1. Volume 59, Issue 6, Pages 2164-2173 (June 2001)
Chronic aristolochic acid toxicity in rabbits: A model of Chinese herbs nephropathy? 
Jean-Pierre Cosyns, Jean-Paul Dehoux, Yves Guiot, Rose-Marie Goebbels, Annie Robert, Alfred M. Bernard, Charles Van Ypersele De Strihou 
Kidney International 
Volume 59, Issue 6, Pages (June 2001)
DOI: /j x
Copyright © 2001 International Society of Nephrology Terms and Conditions

2. Figure 1
Extensive, hypocellular interstitial fibrosis and tubular atrophy in outer cortical labyrinth (OCL) of a female New Zealand white rabbit injected with intraperitoneal 0.1mg aristolochic acid (AA)/kg/day for 17 months (hematoxylin and eosin stain, ×260).
Kidney International  , DOI: ( /j x)
Copyright © 2001 International Society of Nephrology Terms and Conditions

3. Figure 2
Patterns of renal interstitial fibrosis in female New Zealand white rabbits after a 17- to 21-month regimen of an intraperitoneal injection of 0.1 AA mg/kg/day. (A) Cortical labyrinth and medullary rays (MR) (arrows) of control rabbit. (B) Interstitial fibrosis mainly in MR (arrows; pattern I) and mildly in outer cortical labyrinth (OCL) (full arrowheads). (C) Extensive interstitial fibrosis and tubular atrophy in MR, moderate and mild fibrosis in OCL (full arrowheads) and in inner cortical labyrinth (ICL) (hollow arrowhead), respectively (pattern II). (D) Severe interstitial fibrosis and tubular atrophy in MR and OCL, moderate fibrosis in ICL (hollow arrowheads; pattern III) (hematoxylin and eosin stain, ×4).
Kidney International  , DOI: ( /j x)
Copyright © 2001 International Society of Nephrology Terms and Conditions

4. Figure 3
(A) Schematic drawing of the location in the cortex of the various segments of superficial and deep nephrons in normal rabbits. (B) The increase of hypocellular interstitial fibrosis is schematically represented through three patterns in the kidneys of female New Zealand White rabbits (N = 11) injected with intraperitoneal 0.1mg AA/kg/day 5/7 days during 17 (N = 5) and 21 (N = 6) months. Fibrosis is indicated by shades of gray. Abbreviations are: OCL, outer cortical labyrinth; ICL, inner cortical labyrinth; MR, medullary ray; OS/ICL, outer stripe of outer medulla (OM) in front of ICL; OS/MR, outer stripe of outer medulla (OM) in front of MR; IS, inner stripe of OM; IM, inner medulla. In all patterns, MRs are extensively involved. In pattern I, interstitial fibrosis is almost restricted to MR and corresponding OM. This suggests that the initial insult occurs in the straight part (S3 segment) of the proximal tubules of the superficial nephrons. In pattern II, fibrosis has extended to the OCL, suggesting subsequent involvement of the convoluted parts (S1 and S2 segments) of the proximal tubules of the superficial nephrons. In pattern III, there is additional involvement of ICL and corresponding OM suggesting extension of injury to the proximal tubules of deep nephrons. This increase of interstitial fibrosis provides an explanation for its decreasing intensity from the OCL to the ICL in these animals.
Kidney International  , DOI: ( /j x)
Copyright © 2001 International Society of Nephrology Terms and Conditions

5. Figure 4
Cortex of patient with end-stage Chinese herbs nephropathy. Hypocellular interstitial fibrosis and tubular atrophy are more marked in medullary ray (MR) (arrows) and the outer cortical labyrinth (OCL). Tubules are still visible in the inner cortical labyrinth (ICL) (arrowhead). This picture is to be compared with Figure 2d. Periodic acid-Schiff stain, ×30.
Kidney International  , DOI: ( /j x)
Copyright © 2001 International Society of Nephrology Terms and Conditions