Presentation is loading. Please wait.

Presentation is loading. Please wait.

Midkine is expressed by infiltrating macrophages in in-stent restenosis in hypercholesterolemic rabbits  Hiroshi Narita, MD, Sen Chen, PhD, Kimihiro Komori,

Published bySharlene Ferguson Modified over 6 years ago

Similar presentations

Presentation on theme: "Midkine is expressed by infiltrating macrophages in in-stent restenosis in hypercholesterolemic rabbits  Hiroshi Narita, MD, Sen Chen, PhD, Kimihiro Komori,"— Presentation transcript:

1 Midkine is expressed by infiltrating macrophages in in-stent restenosis in hypercholesterolemic rabbits  Hiroshi Narita, MD, Sen Chen, PhD, Kimihiro Komori, MD, PhD, Kenji Kadomatsu, MD, PhD  Journal of Vascular Surgery  Volume 47, Issue 6, Pages (June 2008) DOI: /j.jvs Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 Induction of neointimal hyperplasia by bare metal stent implantation. A, The arrows in the (a) plain radiographs and (b) angiography after stent implantation into the right iliac artery indicate the stented lesion. B, Neointimal hyperplasia was induced moderately in a (a) rabbit fed a normal diet, but was markedly induced in a (b) rabbit fed a hypercholesterol diet. Bar, 500 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Midkine expression during neointima formation. Right iliac arteries of hypercholesterolemic rabbits were obtained at the indicated number of days after stenting. The graphs show relative densitometric density ratios presented as the average ± SE (n = 4). *P < .01; **P < (Student t test). Western blot and reverse transcription–polymerase chain reaction (RT-PCR) data were obtained from two individual animals, respectively, at each time point (except for control and day 7: n = 4). Because the results of each set of experiments were similar, representative data are shown. A, Midkine protein expression revealed by Western blot analysis. B, Midkine messenger RNA expression estimated by RT-PCR. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Protein localization in neointima hyperplasia. Immunohistochemistry data were obtained from three individual animals at each time point. Because the results of each set of experiments were similar, representative data are shown. A, Chicken antihuman midkine antibody (upper) or control chicken immunoglobulin (Ig) Y was used as the first antibody to localize midkine protein expression in an artery obtained 14 days after operation. Note that IgY did not show any signals. Bar, 100 μm. B, The indicated antibodies were used to localize their target proteins in the specimen. The midkine protein localization overlapped that of macrophages (RAM11). Bars, 100 μm. α-SMA, α-Smooth muscle actin. C, The double immunofluorescent staining for midkine and macrophage was performed on specimens 7 days after stenting. S indicates stent strut. Bar, 20 μm. D, Midkine protein was localized to the endothelium. Magnified photos of B, c and B, l are shown. Bar, 20 μm. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Midkine expression during differentiation of monocytic cells. A, Phase-contrast photos are shown. THP-1 cells were treated with either (left) dimethyl sulfoxide (DMSO) or (right) phorbol myristate acetate (PMA) for 24 hours. B, Western blot analysis for midkine protein expression. The graph at right shows the average ± SE values of the relative densitometric density ratio (n = 4). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

6 Fig 5 Effect of oxided low-density lipoprotein cholesterol (oxLDL) on midkine expression in macrophages. A, Peritoneal macrophages were exposed to 100 μg/mL of LDL or oxLDL for 24 hours. Cells were then stained with oil red O to detect lipid droplets in the cell. Note that oxLDL was significantly more endocytosed by the cell. B, Midkine expression was estimated by Western blot analysis. The right graph shows the average ± SE values of the relative densitometric density ratio (n = 4). There were no significant differences among these three groups. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2008 The Society for Vascular Surgery Terms and Conditions

Download ppt "Midkine is expressed by infiltrating macrophages in in-stent restenosis in hypercholesterolemic rabbits  Hiroshi Narita, MD, Sen Chen, PhD, Kimihiro Komori,"

Similar presentations

ACE Inhibitor Quinapril Reduces the Arterial Expression of NF-κB-Dependent Proinflammatory Factors but not of Collagen I in a Rabbit Model of Atherosclerosis 

ACE Inhibitor Quinapril Reduces the Arterial Expression of NF-κB-Dependent Proinflammatory Factors but not of Collagen I in a Rabbit Model of Atherosclerosis 
Tacrolimus-eluting stent inhibits neointimal hyperplasia via calcineurin/NFAT signaling in porcine coronary artery model  Narisato Hamada, Masaaki Miyata,

Tacrolimus-eluting stent inhibits neointimal hyperplasia via calcineurin/NFAT signaling in porcine coronary artery model  Narisato Hamada, Masaaki Miyata,
Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia  Go Kuwahara,

Vascular endothelial growth factor-C derived from CD11b+ cells induces therapeutic improvements in a murine model of hind limb ischemia  Go Kuwahara,
The effects of doxycycline and micronized purified flavonoid fraction on human vein wall remodeling are not hypoxia-inducible factor pathway-dependent 

The effects of doxycycline and micronized purified flavonoid fraction on human vein wall remodeling are not hypoxia-inducible factor pathway-dependent 
Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.

Dipeptidyl peptidase 4 inhibitor reduces intimal hyperplasia in rabbit autologous jugular vein graft under poor distal runoff  Akio Koyama, MD, Kimihiro.
Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 

Accelerated aneurysmal dilation associated with apoptosis and inflammation in a newly developed calcium phosphate rodent abdominal aortic aneurysm model 
Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps  Rahmi Oklu,
Direct stenting limits sirolimus-eluting stent edge neointimal thickening  Patrice Guérin, MD, PhD, Yann Gouëffic, MD, PhD, Marie-Françoise Heymann, MD,

Direct stenting limits sirolimus-eluting stent edge neointimal thickening  Patrice Guérin, MD, PhD, Yann Gouëffic, MD, PhD, Marie-Françoise Heymann, MD,
Wei Zhou, MD, Hong Chai, MD, PhD, Andy Courson, BS, Peter H

Wei Zhou, MD, Hong Chai, MD, PhD, Andy Courson, BS, Peter H
Undifferentiated mesenchymal stem cells seeded on a vascular prosthesis contribute to the restoration of a physiologic vascular wall  Alain Mirza, MD,

Undifferentiated mesenchymal stem cells seeded on a vascular prosthesis contribute to the restoration of a physiologic vascular wall  Alain Mirza, MD,
Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 

Yi-kuan Chen, MD, Xue-mei Jiang, MSc, Jian-ping Gong, MD 
Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,

Elevation of hemopexin-like fragment of matrix metalloproteinase-2 tissue levels inhibits ischemic wound healing and angiogenesis  April E. Nedeau, MD,
Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms  Adam J. Doyle, MD, Eileen M. Redmond,

Differential expression of Hedgehog/Notch and transforming growth factor-β in human abdominal aortic aneurysms  Adam J. Doyle, MD, Eileen M. Redmond,
Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway  Hidenori.

Sustained orbital shear stress stimulates smooth muscle cell proliferation via the extracellular signal-regulated protein kinase 1/2 pathway  Hidenori.
Growth hormone–releasing peptide ghrelin inhibits homocysteine-induced endothelial dysfunction in porcine coronary arteries and human endothelial cells 

Growth hormone–releasing peptide ghrelin inhibits homocysteine-induced endothelial dysfunction in porcine coronary arteries and human endothelial cells 
The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization.

The therapeutic effect of vascular endothelial growth factor gene- or heme oxygenase-1 gene-modified endothelial progenitor cells on neovascularization.
Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats  Zhenhai Yu, MD, Keisuke.

Endogenous superoxide dismutase activation by oral administration of riboflavin reduces abdominal aortic aneurysm formation in rats  Zhenhai Yu, MD, Keisuke.
Xue Ma, MD, Jeffrey D. Pearce, MD, David B. Wilson, MD, William P

Xue Ma, MD, Jeffrey D. Pearce, MD, David B. Wilson, MD, William P
Hemin prevents in-stent stenosis in rat and rabbit models by inducing heme-oxygenase- 1  Jean-Marc Hyvelin, PhD, Blandine Maurel, MSc, MD, Rustem Uzbekov,

Hemin prevents in-stent stenosis in rat and rabbit models by inducing heme-oxygenase- 1  Jean-Marc Hyvelin, PhD, Blandine Maurel, MSc, MD, Rustem Uzbekov,
Autologous human plasma in stem cell culture and cryopreservation in the creation of a tissue-engineered vascular graft  Ping Zhang, PhD, Aleksandra Policha,

Autologous human plasma in stem cell culture and cryopreservation in the creation of a tissue-engineered vascular graft  Ping Zhang, PhD, Aleksandra Policha,

Similar presentations

Ads by Google