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Xenotransplantation by injection of a suspension of isolated preantral ovarian follicles and stroma cells under the kidney capsule of nude mice Jan M.J. Aerts, Ph.D., Belen Martinez-Madrid, Ph.D., Jo L.M.R. Leroy, Ph.D., Stefan Van Aelst, Ph.D., Peter E.J. Bols, Ph.D. Fertility and Sterility Volume 94, Issue 2, Pages (July 2010) DOI: /j.fertnstert Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 Xenotransplantation of isolated preantral follicles to the kidney capsule of a nude mouse. The pipette tip was introduced through a capsulotomy to deposit the follicular suspension (arrow). Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions
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Figure 2 Comparison between the follicular stages (primordial, intermediate, primary, and secondary) in bovine fetal and calf ovarian control tissue and grafted isolated preantral follicles, xenotransplanted for 14 days to the renal capsule of nude mice. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions
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Figure 3 Histologic sections of bovine isolated preantral follicle grafts, xenotransplanted under the kidney capsule of nude mice for 14 days. (A) Hematoxylin and eosin section exhibiting murine renal tissue and two primary follicles (arrows) in the graft (dashed area). Original magnification ×100. (B) Immunohistochemical staining for proliferating cell nuclear antigen of a cluster of intermediary and primary follicles. Original magnification ×200. (C) Immunohistochemical staining for proliferating cell nuclear antigen showing two secondary follicles. Original magnification ×100. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions
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