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Pneumocystis jirovecii multilocus genotyping in pooled DNA samples: a new approach for clinical and epidemiological studies  F. Esteves, J. Gaspar, B.

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Presentation on theme: "Pneumocystis jirovecii multilocus genotyping in pooled DNA samples: a new approach for clinical and epidemiological studies  F. Esteves, J. Gaspar, B."— Presentation transcript:

1 Pneumocystis jirovecii multilocus genotyping in pooled DNA samples: a new approach for clinical and epidemiological studies  F. Esteves, J. Gaspar, B. de Sousa, F. Antunes, K. Mansinho, O. Matos  Clinical Microbiology and Infection  Volume 18, Issue 6, Pages E177-E184 (June 2012) DOI: /j x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

2 FIG. 1 Graphic representation of Pneumocystis jirovecii single nucleotide polymorphism (SNP) allele relative frequencies distribution among the different pools studied—A1, AIDS-related Pneumocystis pneumonia (PcP) episodes with low parasite burden; A2, AIDS-related PcP episodes with moderate parasite burden; A3, AIDS-related PcP episodes with high parasite burden; D, AIDS-related PcP cases presenting positive follow-up; E, AIDS-related PcP cases presenting negative follow-up). The relative frequency of each SNP was calculated dividing the maximum height values of fluorescence peaks observed in the single base extension products by the reference fluorescence values observed in the positive control. Clinical Microbiology and Infection  , E177-E184DOI: ( /j x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions


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