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Chimerism Analysis in the Pediatric Setting

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Presentation on theme: "Chimerism Analysis in the Pediatric Setting"— Presentation transcript:

1 Chimerism Analysis in the Pediatric Setting
Susanne Kricke, Lana Mhaldien, Rozendo Fernandes, Charizel Villanueva, Alistair Shaw, Paul Veys, Stuart Adams  The Journal of Molecular Diagnostics  Volume 20, Issue 3, Pages (May 2018) DOI: /j.jmoldx Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2 Figure 1 Example data plots from randomly selected samples of whole blood (WB); bone marrow (BM); and cell fractions CD3+, CD15+, and CD19+, comparing quality of peak height and signal strength of the short tandem repeat markers vWA and D3S1358. A and B: vWA marker using the PowerPlex 16 System (A) and the GenePrint 24 System (B) (Promega UK, Southampton, UK). C and D: D3S1358 marker using PowerPlex 16 (C) and GenePrint 24 (D). al, allele; ar, area; sz, size. The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3 Figure 2 Results of chimerism analysis of samples of bone marrow; whole blood (including samples from the UK National External Quality Assessment Scheme); and cell fractions CD3+, CD15+, and CD19+, and their corresponding DNA samples, with line of best fit (dotted lines). The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4 Figure 3 Results of purity assessment for cell fractions CD3+ and CD19+, and their corresponding DNA samples, with line of best fit (dashed line). The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx ) Copyright © 2018 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions


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