1. (A) Six missense mutations in six essential genes that are not in annotated functional domains.
(A) Six missense mutations in six essential genes that are not in annotated functional domains. (B) One gene model (let-522/M05B5.2) that has a missense mutation h240 (C68Y) that does not reside in an annotated domain (Chu et al. 2014). The gene model was created using the Perl module FeatureStack. Exons are drawn to scale relative to each other in gray boxes; black lines with triangle shapes represent introns, which are not drawn to scale; white boxes represent UTRs. Blue bars indicate the missense mutation. Multiple alignments were performed between the essential genes and their orthologs in 25 other nematodes and four model organisms (Homo sapiens, Drosophila melanogaster, Mus musculus, and Danio rerio). However, no orthologs were found in the four model organisms. Left columns show the names of nematode species. The alignment figure shows a fragment of 100 amino acids. The mutated amino acid (C68Y) as indicated by the black arrow is in the center with 50 amino acids upstream and 49 amino acids downstream. The alignment color scheme is based on “ClustalX,” in which the color of a symbol depends on the residue type and the occurrence frequency in one column. Black boxes highlight the input genes with both wild type and mutant copy.
Zhaozhao Qin et al. G3 2018;8:
©2018 by Genetics Society of America