1. Jianjiang Xu, Wenqing Zhu, Jiaxu Hong
Investigation of chlorouine-induced keratopathy on in vivo confocal microscopy
Jianjiang Xu, Wenqing Zhu, Jiaxu Hong
Eye & ENT Hospital
Fudan University
Shanghai, China

2. None of commercial relationships with authors.
Title: Investigation of chlorouine-induced keratopathy on in vivo confocal microscopy
Paper ID: 365
Disclosure Block:
None of commercial relationships with authors.

3. Outlines Background Objective Methods Results Conclusion
Eye & ENT Hospital

4. Background
Chloroquine (CHQ) was used successfully for the treatment of several chronic autoimmune diseases
However, a variety of side effects have been reported, of which ophthalmic toxicity is of the utmost importance.
Chloroquine
Corneal Deposits

5. Background
In vivo confocal microscopy(CM) has dramatically changed the ophthalmic clinical landscape, allowing a non-invasive approach to in vivo visualize cornea at a magnification of up to ×700
50 µm
Cornea
Epithelium
Stroma
Endothelium

6. Objective
To investigate morphological changes of the cornea by CM in patients diagnosed as rheumatoid arthritis and treated with CHQ

7. Methods Subjects Examination:
30 patients with rheumatoid arthritis were enrolled
20 were treated with CHQ
the rest served as control
Examination:
Bilateral eyes were examined by slitlamp biomicroscope and CM
CHQ deposits and corneal cellular densities were analyzed

8. Results Deposits No deposit was observed by SLB examination.
Under CM, CHQ deposits were
observed in 28 (70.0%) of 40
eyes in patients
CM deposits demonstrate different
characteristic features of the CHQ
between two groups.

9. Results Cellular density
The cellular density of each layer had no significant difference between two groups except the density of anterior stromal keratocytes.

10. Conclusion
CM is a useful tool for the investigation of keratopathy induced by CHQ treatment
CHQ deposits were mainly identified in corneal epithelium and stroma
They might correspond to intracellular lysosome-like corpuscles which could not be metabolised
Abnormal microenvironment on basal epithelial cells leads to a vicious cycle on the maturation of epithelial cells.

11. Acknowledgement Team Prof. Jianjiang Xu Wenqing Zhu M.D.
Jiaxu Hong M.D.
Anji Wei M.D.
Gang Li Ph.D
Grants: This work was supported partly by grants from the Key Clinic Medicine Research Program, the Ministry of Health, China ( ).

12. 最后非常荣幸这次有机会给各位专家介绍我们的研究项目.
Thank you