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First next-generation sequencing full-genome characterization of a hepatitis C virus genotype 7 divergent subtype M. Salmona, A. Caporossi, P. Simmonds, M.-A. Thélu, K. Fusillier, S. Mercier-Delarue, N. De Castro, J. LeGoff, M.-L. Chaix, O. François, F. Simon, P. Morand, S. Larrat, S. Maylin Clinical Microbiology and Infection Volume 22, Issue 11, Pages 947.e1-947.e8 (November 2016) DOI: /j.cmi Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions
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Fig. 1 Maximum-likelihood phylogeny estimated from HCV complete coding region. Sequence analysed corresponded to near-full-length coding region. For all genotypes, lowest common branch shared by all subtypes is labelled with its bootstrap value (n = 1000). BAK1 isolate described in this study is in boldface type. Scale bar represents 0.1 nucleotide substitution per site. HCV, hepatitis C virus. Clinical Microbiology and Infection , 947.e1-947.e8DOI: ( /j.cmi ) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions
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Fig. 2 Maximum-likelihood phylogeny estimated from HCV NS5B region. Phylogeny was reconstructed using NS5B sequences (239 nt long) of genomes included in Fig. 1 and of isolates QC272, IG93306 and IG Branch with bootstrap support of >60% are labelled with their bootstrap support values. BAK1 and VELA isolates described in this study are in boldface type. Genotypes 1 to 6 have been collapsed for clarification. Phylogeny is midpoint rooted. Scale bar represents 0.1 nucleotide substitution per site. HCV, hepatitis C virus. Clinical Microbiology and Infection , 947.e1-947.e8DOI: ( /j.cmi ) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions
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Fig. 3 Comparison of distances between BAK1 (HCV-7x) and HCV-7a with intra- and intergenotype distance of other HCV genotype. Sequence divergence between BAK1 (HCV-7x) and HCV-7a (black line) was compared to intra- and intergenotype distances of HCV genotypes 1 to 7. Mean distances were calculated using sliding window of 300 bases incrementing by 15 bases across genome using program Sequence Distance in SSE software. HCV, hepatitis C virus. Clinical Microbiology and Infection , 947.e1-947.e8DOI: ( /j.cmi ) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions
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Fig. 4 (A) Bootscan and (B) Grouping scan of BAK1 as reference sequence with representative complete genome sequences of HCV genotypes 1 to 7. Analysis by both methods used sliding window of 300 bases, incrementing by 15 bases across genome. Because genotype 7a was represented by single sequence in analysis, small group correction factor was selected in Grouping scan. HCV, hepatitis C virus. Clinical Microbiology and Infection , 947.e1-947.e8DOI: ( /j.cmi ) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions
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Clinical Microbiology and Infection 2016 22, 947. e1-947. e8DOI: (10
Clinical Microbiology and Infection , 947.e1-947.e8DOI: ( /j.cmi ) Copyright © 2016 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions
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