1. Nanotechnology 22 (2011) 245101 (8pp)
Transport of single cells using an actin bundle–myosin bionanomotor transport system
Hideyo Takatsuki, Hideyuki Tanaka, Kevin M Rice, Madhukar B Kolli, Siva K Nalabotu, Kazuhiro Kohama, Parviz Famouri and Eric R Blough
Marshall University, Huntington, USA
Gunma University Graduate School of Medicine, Japan
West Virginia University, Morgantown, USA

2. Introduction
Actin are muscle protein localized in the myofibrils; acting along with myosin, it is responsible for contraction and relaxation of muscle. It occurs in globular (G-actin) and fibrous (F-actin) forms.
Individual subunits of microfilaments are known as globular actin (G-actin). G-actin subunits assemble into long filamentous polymers called F-actin

3. Nitro-benzoxadiazole
-NBD
Green fluorescent protein
- GFP
Biotinylated liposome (labeled with NBD; green) (a) and E. coli cell (expressing GFP; green) (b) that have been bound to biotin-labeled actin bundle (red) via neutravidin linker (blue).

4. Morphology
150 nm
300 nm
microne
TEM
-ve Staining

5. Motility of liposome on actin filament
Image series (a)
U-turn
Overlay image(b)
Video

6. Motility of liposome on actin bundle
Image series (a)
Arrows and arrowheads point
to liposomes and bundles
Actin bundle
Overlay image(b)
Video

7. Motility of cell on actin bundle
Arrows and arrowheads point to E.coli cell and bundles.
Video

8. Velocity
3.9±
0.2
3.0±
0.2
4.0±
0.2
3.8±
0.2
3.4±
0.1
2.4±
0.1
2.3±
0.1
1.8±
0.1
2.1±
0.1
1.7±
0.1

9. Linearity of translocation,
87± 2
85± 4
77± 4
53± 4
56± 5
39± 3
39± 2
27± 4
48± 4
40± 11

10. Unloading UV reactive chemical linkages in catalytic motors
DNA tethers alone
Combination of liposome with MT-Kinesin system
Triton X-100 added

11. Conclusion
F-actin and actin bundles can be used to transport liposomes over a myosin motor-patterned surface while actin bundles can only have the capacity to transport E. coli cells.
Actin bundles have advantageous over F-actin systems for the nanotransport of cargo given that the bundles exhibit greater efficiency of translational movement, higher velocity of translocation and the ability to move larger loads over a greater distance.
Triton X-100 could be used as a means of introducing liposome rupture and presumably cargo unloading following the transport of liposomes by the actin–myosin motor platform.
thank you