1. (30% amylopectin, high amylose)
Table S1. Utilization of different starches by five species of human colonic bacteria1. Growth was on YCFAS medium, except for R. bromii which was grown in M2S medium (see main text and Fig.1 for details).
Bacterial strains3
Type of Starch
Treatment2
B.theta
5482
E.rectale
A1-86
R.bromii
L2-63
B.adol
L2-32
B.breve
202134
Maize
(100% amylopectin)
Sigma
S9679 A
93.9 ± 0.1a
81.3 ± 1.1c
76.0 ± 1.2d
86.6 ± 0.4b
85.5 ± 0.7 B
93.0 ± 0.7a
83.0 ± 0.8bc
75.6 ± 5.5c
85.9 ± 0.6ab
84.6 ± 0.4 R
53.1 ± 16.4ab
27.7 ± 12.2b
68.0 ± 16a
84.2 ± 0.7a
72.3 ± 4.2
Wheat
(75% amylopectin)
S5127
87.9 ± 0.7a
82.5 ± 0.3a
70.0 ± 4.5b
86.7 ± 0.3a
84.7 ± 0.9
75.2 ± 3.5bc
81.9 ± 1.2ab
71.8 ± 4.2c
86.2 ± 0.4a
84.2 ± 0.2
37.8 ± 3.0b
18.2 ± 17.6b
82.9 ± 8.1a
84.7 ± 0.5a
77.9 ±0.5
Rice
(~80% amylopectin)
S7260
92.0 ± 0.4a
81.8 ± 1.7c
75.7 ± 1.5d
87.2 ± 0.8b
84.7 ± 0.3
80.4 ± 3.2a
80.3 ± 1.7a
69.4 ± 3.3b
86.4 ± 0.6a
84.2 ± 1.3
29.3 ± 1.8c
28.2 ± 3.4c
61.5 ± 7.1b
83.8 ± 0.2a
79.8 ± 0.4
(30% amylopectin, high amylose)
S4180
54.2 ± 1.7c
60.3 ± 2.1c
68.9 ± 3.3b
75.8 ± 1.2a
66.4 ± 1.3
29.3 ± 9.2c
31.7 ± 9.1bc
61.2 ± 0.5a
48.8 ± 1.2ab
46.6 ±1.4
0.30 ± 6.2b
11.0 ± 4.3b
54.3 ± 17.1a
43.8 ± 3.1a
36.1 ± 4.7
(high amylose)
RS2
HM-958
57.5 ± 3.3c
60.3 ± 2.3bc
77.4 ± 8.3a
71.1 ± 2.0ab
Nt5
20.2 ± 2.9c
39.7 ± 4.3b
72.0 ± 3.2a
67.9 ± 4.2a Nt
17.8 ± 3.7c
7.50 ± 6.9c
69.5 ± 7.5a
53.3 ± 0.3b
HM-240
65.6 ± 3.9a
58.1 ± 2.4b
64.0 ± 1.5ab
70.4 ± 0.7a
11.0 ± 12.9c
27.4 ± 2.7bc
44.3 ± 8.7ab
61.0 ± 3.4a
0.25 ± 3.2b
0.53 ± 3.6b
39.1 ± 4.7a
26.6 ± 6.0a
(retrograded)
RS3
NL-
330
47.2 ± 0.9b
52.2 ± 1.4b
71.4 ± 4.7a
71.4 ± 1.4a
7.00 ± 8.1c
29.0 ± 2.7b
46.6 ± 6.5a
51.5 ± 0.4a
4.80 ± 7.9b
16.5 ± 1.0b
43.1 ± 5.7a
36.9 ± 6.3a
1- values shown in the table are percentage of starch utilization ± Std. dev after 72h incubation.
2- three treatments were compared: A, autoclaved; B, boiled and R, raw.
3- the difference in starch utilization by four bacteria strains was compared. Different lowercase
letters indicate significant differences (p<0.05) in each row. No significant difference between bacteria
strains that share the same letters (p>0.05).
4- B. breve was not included for statistical analysis.
5- Nt, not tested