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Airway epithelial cells from asthmatic children differentially express proremodeling factors  Jesus M. Lopez-Guisa, PhD, Claire Powers, BA, Daniele File,

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Presentation on theme: "Airway epithelial cells from asthmatic children differentially express proremodeling factors  Jesus M. Lopez-Guisa, PhD, Claire Powers, BA, Daniele File,"— Presentation transcript:

1 Airway epithelial cells from asthmatic children differentially express proremodeling factors 
Jesus M. Lopez-Guisa, PhD, Claire Powers, BA, Daniele File, BS, Elizabeth Cochrane, BS, Nathalia Jimenez, MD, MPH, Jason S. Debley, MD, MPH  Journal of Allergy and Clinical Immunology  Volume 129, Issue 4, Pages e6 (April 2012) DOI: /j.jaci Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 TGF-β2 concentrations in ALI cultures from bronchial epithelial cells from healthy subjects (Healthy BE), bronchial epithelial cells from asthmatic patients (Asthma BE), bronchial epithelial cells from atopic nonasthmatic patients (Atopic BE), nasal epithelial cells from healthy subjects (Healthy NE), nasal epithelial cells from asthmatic patients (Asthma NE), and nasal epithelial cells from atopic nonasthmatic patients (Atopic NE). *Three-way group comparison of Asthma BE versus Healthy BE and Atopic BE: P = .02 (Kruskal-Wallis test). Comparison of Asthmatic BE with Healthy BE: P = .01 (Mann-Whitney test). Comparison of Asthmatic BE with Atopic BE: P = .02 (Mann-Whitney test). **Three-way group comparison of Asthma NE versus Healthy NE and Atopic NE: P = .05 (Kruskal-Wallis test). Comparison of Asthmatic NE with Healthy NE: P = .04 (Mann-Whitney test). Comparison of Asthmatic NE to Atopic NE: P = .04 (Mann-Whitney test). Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 VEGF concentration in ALI cultures from bronchial epithelial cells from healthy subjects (Healthy BE), bronchial epithelial cells from asthmatic patients (Asthma BE), bronchial epithelial cells from atopic nonasthmatic patients (Atopic BE), nasal epithelial cells from healthy subjects (Healthy NE), nasal epithelial cells from asthmatic patients (Asthma NE), and nasal epithelial cells from atopic nonasthmatic patients (Atopic NE). *Three-way group comparison of Asthma BE versus Healthy BE and Atopic BE: P < .001 (1-way ANOVA). Comparison of Asthmatic BE with Healthy BE: P = .003 (unpaired t test). Comparison of Asthmatic BE with Atopic BE: P = .004 (unpaired t test). **Three-way group comparison of Asthma NE versus Healthy NE and Atopic NE: P < .05 (Kruskal-Wallis test). Comparison of Asthmatic NE with Healthy NE: P < .001 (Mann-Whitney test). Comparison of Asthmatic NE with Atopic NE: P = .001 (Mann-Whitney test). Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig 3 Correlation between bronchial and nasal epithelial cell ALI cultures for production of TGF-β2 (A) and VEGF (B). Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig 4 Periostin and ADAM33 mRNA expression in asthmatic bronchial (A) and nasal (B) epithelial cell ALI cultures compared with expression in cultures from atopic nonasthmatic and healthy children normalized to the housekeeping gene GAPDH. Fig 4, A: Periostin overall group differences, P = .001; asthmatic patients versus healthy subjects, P = ADAM33 showed no significant between-group differences. Fig 4, B: Periostin overall group differences, P = .05; asthmatic patients versus healthy subjects, P = ADAM33 showed no significant differences between groups. C, Periostin, ADAM33, and GAPDH amplicons determined by means of gel electrophoresis. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E1 Hematoxylin and eosin–stained bronchial epithelial cells from an asthmatic patient differentiated at an ALI. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E2 Change in TGF-β2 concentrations in ALI conditioned culture media from healthy bronchial (A), asthmatic bronchial (B), and atopic nonasthmatic bronchial (C) epithelial cells after stimulation with IL-4 and IL-13. Change in VEGF concentrations in ALI conditioned culture media from healthy bronchial (D), asthmatic bronchial (E), and atopic nonasthmatic bronchial (F) epithelial cells after stimulation with IL-4 and IL-13. Analyses were conducted with paired t tests or Wilcoxon matched-pairs test, as appropriate. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

8 Fig E3 Production of TGF-β2 (A) and VEGF (B) in bronchial epithelial cell ALI cultures from asthmatic children who reported use of ICSs at the time of airway brushings (+ICS) was similar to production in cells from asthmatic children who were not using inhaled steroids (−ICS). Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

9 Fig E4 Periostin expression (green box) was 2.4-fold higher in bronchial epithelial cells from asthmatic patients stimulated with IL-4 and IL-13 compared with that seen in unstimulated cells from asthmatic patients (A; P = .046). There was no significant change in periostin expression after IL-4 and IL-3 stimulation by bronchial epithelial cells from healthy subjects (B; P = .5) or atopic nonasthmatic patients (C; P = .4). ADAM33 expression (blue box) by AECs from asthmatic (Fig E4, A), healthy (Fig E4, B), or atopic nonasthmatic (Fig E4, C) children did not change in response to IL-4 and IL-13 stimulation. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

10 Fig E5 Periostin expression (green box) was 3.3-fold higher in asthmatic bronchial epithelial cells from children not using inhaled steroids (−ICS) than expression in healthy children (A; P = .003). Periostin expression in asthmatic children who were using inhaled steroids (+ICS) was 4-fold higher than expression in cells from healthy children (B; P = .001). ADAM33 (blue box) was not differentially expressed by either −ICS (Fig E5, A; P = .2) or +ICS (Fig E5, B; P = .9) asthmatic children in comparison with healthy children. Journal of Allergy and Clinical Immunology  , e6DOI: ( /j.jaci ) Copyright © 2012 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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