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Measuring small volumes
June 2013 Paul Beaumont
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[Evaluating experimental procedures p. 50]
Learners should be able to…: … identify sources of error in measurements and observations identify and comment on the reliability of results ...
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Skills of scientific experimentation, investigation and enquiry
Evaluating (p 8) Learners should be able to…: … identify sources of error in measurements and observations identify and comment on the reliability of results ...
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Syringes / pipettes Variable volume 0 - 20 μL, 0 - 200 μL, 0 - 1000 μL
~ £200 per syringe
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Syringes / pipettes Single volume
5 μL, 10 μL, 20 μL, 25 μL, 50 μL and 100 μL £16 per syringe
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Making your own microsyringe
Accuracy? Precision? Cost?
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Equipment
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Equipment 10 mL 20 mL 50 mL 100 mL 200 mL
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Operation / activity Draw up some air - ~ 100 mL No more than 200 mL!
Measure mass of empty weighing boat Take ca. 150 mL - expel excess until 100 mL left Repeat 9 times - measure mass Record mass on board Repeat using 50 mL increments
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