1. Volume 141, Issue 1, Pages 227-236 (July 2011)
Twin Study Indicates Loss of Interaction Between Microbiota and Mucosa of Patients With Ulcerative Colitis 
Patricia Lepage, Robert Häsler, Martina E. Spehlmann, Ateequr Rehman, Aida Zvirbliene, Alexander Begun, Stephan Ott, Limas Kupcinskas, Joël Doré, Andreas Raedler, Stefan Schreiber 
Gastroenterology 
Volume 141, Issue 1, Pages (July 2011)
DOI: /j.gastro
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2. Figure 1
Sørensen similarity index based on qualitative analysis of phylotypes. (A) Phylotypes were compared between individuals 2 by 2 (or between siblings from each twin pair) within each clinical group (ie, aM01 vs bM01). Unrelated healthy individuals (n = 22); Hli-DZ, healthy dizygotic twins (n = 11 pairs); Hli-MZ, healthy monozygotic twins (n = 7 pairs); MZ-uc, monozygotic twins discordant for UC (n = 8 pairs). P, P value using an unpaired Student t test; P value in black, comparison with Hli-DZ; P value in red, comparison with unrelated individuals. (B) Sørensen similarity index based on qualitative analysis of phylotypes. Phylotypes were pooled for half of the overall pairs for each clinical group and compared with the other half (ie, aM vs bM).
Gastroenterology  , DOI: ( /j.gastro )
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3. Figure 2
Estimated bacterial diversity in the 4 clinical groups using the Chao1 richness estimator. Chao1 is a nonparametric estimator for species richness, taking into account the observed phylotypes number and the number of singletons and doubletons within the population. Light-colored areas represent the 95% confidence intervals computed with DOTUR. H-ger, healthy German individuals (n = 10); H-lith, healthy Lithuanian individuals (n = 10; randomly chosen samples to estimate phylotypes richness); Hu, healthy siblings from the UC discordant twin pairs (n = 8); UC, UC siblings from the UC discordant twin pairs (n = 8).
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2011 AGA Institute Terms and Conditions

4. Figure 3
Phyla repartition for each clinical group. Clinical groups are as defined in the legend to Figure 2. Box plots represent 25th percentile, median, and 75th percentile. Whiskers represent minimum and maximum values. Empty circles represent values of outliers. As pooled samples from 10 individuals were analyzed within the healthy German cohort, only means were computed that are as follows: Firmicutes, 42.94%; Bacteroidetes, 41.98%; Proteobacteria, 12.30%; and Actinobacteria, 2.77%.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2011 AGA Institute Terms and Conditions

5. Figure 4
PCA of microbiota phylotypes and host transcripts within UC discordant twin pairs. (A) PCA and clustering using Ward's minimum variance method with correlation distances analysis of the phylotypes repartition between Hu (white circles, healthy siblings) and UC (gray circles, UC siblings) patients. These 2 components explain 49.73% of the variability. Statistical clustering using Ward's minimum variance method is represented by ellipses on the PCA. (B) PCA of the gene expression based on 361 differentially expressed genes between Hu and UC. Hu (white circles), healthy siblings from the UC discordant twin pairs (n = 8); UC (gray circles), UC-affected siblings from the UC discordant twin pairs (n = 8). These 2 components explain 63.52% of the variability. (C) Impact of medical treatment on phylotype composition; interclass analysis of discordant twin pairs. PCA with medical treatment as a clinical factor was performed based on the phylotype abundance. Individuals (gray circles, UC siblings; white circles, unaffected siblings from UC discordant pairs) were clustered (gray ellipses) and the center of gravity computed for each class. P value of the link between medical treatment and phylotype abundance was assessed using a Monte Carlo test (999 replicates). P = AZA, azathioprine; 5-ASA, mesalamine.
Gastroenterology  , DOI: ( /j.gastro )
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6. Figure 5
Correlations between microbiota and gene expression. Transcripts (second column) are organized as Gene Ontology terms for biological processes (first column). Transcripts with no gene symbol are listed with their public reference; a star indicates that this transcript represents the same gene as the previous transcript. Bacterial genera (second row) are classified by phyla (first row). Hli, healthy unrelated individuals from the monozygotic cohort; Hu, healthy individuals from discordant twin pairs (UC); UC, diseased individuals from discordant twin pairs (UC); F, Firmicutes; B, Bacteroidetes; P, Proteobacteria; A, Actinobacteria. Blue squares represent positive correlations (r > 0.45), and yellow squares represent negative correlations (r < −0.45).
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2011 AGA Institute Terms and Conditions