1. Separation of human immunodeficiency virus type 1 from motile sperm by the double tube gradient method versus other methods 
Joseph A Politch, Ph.D., Chong Xu, M.D., Lynne Tucker, M.Sc., Deborah J Anderson, Ph.D. 
Fertility and Sterility 
Volume 81, Issue 2, Pages (February 2004)
DOI: /j.fertnstert

2. Figure 1 Schematic representations of sperm-processing techniques.
Politch. Removal of HIV-1 from motile sperm fraction.Fertil Steril 2004.
Fertility and Sterility  , DOI: ( /j.fertnstert )

3. Figure 2
Comparison of HIV-1 RNA in the motile sperm fractions from single tube gradient (n = 3), swim-up (n = 3), and double tube gradient (n = 3) methods using ISolate sperm separation medium. Data are represented as mean ± standard error HIV-1 RNA copy number per motile sperm fraction.
Politch. Removal of HIV-1 from motile sperm fraction.Fertil Steril 2004.
Fertility and Sterility  , DOI: ( /j.fertnstert )

4. Figure 3
Comparison of HIV-1 RNA in the motile sperm fractions from single tube gradient (n = 6), gradient/swim-up (n = 6), and double tube gradient (n = 6) methods using ISolate sperm separation medium. Data are represented as mean ± standard error HIV-1 RNA copy number per motile sperm fraction.
Politch. Removal of HIV-1 from motile sperm fraction.Fertil Steril 2004.
Fertility and Sterility  , DOI: ( /j.fertnstert )

5. Figure 4
Comparison of sperm yield in the motile sperm fractions from single tube gradient , double tube gradient , and gradient/swim-up methods using ISolate and Percoll sperm separation media. Data are represented as mean ± standard error percent total motile sperm of initial sample. No statistically significant differences in sperm yield were found between ISolate and Percoll.
Politch. Removal of HIV-1 from motile sperm fraction.Fertil Steril 2004.
Fertility and Sterility  , DOI: ( /j.fertnstert )