1. Interleukin-6 Regulation of Direct Lung Ischemia Reperfusion Injury
Alexander S. Farivar, MD, Heather E. Merry, MD, Mauricio J. Fica-Delgado, MD, Anton S. McCourtie, MRCS, Brendan C. Mackinnon- Patterson, BS, Michael S. Mulligan, MD 
The Annals of Thoracic Surgery 
Volume 82, Issue 2, Pages (August 2006)
DOI: /j.athoracsur
Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

2. Fig 1
Electrophoretic mobility shift assay for nuclear factor kappa B (NFκB) nuclear translocation in whole lung homogenates. Lane 2 is the cold competition lane whereas lane 1 represents negative control, unmanipulated lungs. It can be seen that ischemia followed by 15 minutes of reperfusion significantly increases the nuclear translocation of NFκB (lanes 3 and 4) relative to negative controls (p = 0.007), and increases even further after ischemia and 4 hours of reperfusion (p = 0.02) (lane 7). Intratracheal interleukin-6 (IL-6) markedly reduced NFκB transactivation relative to vehicle-treated controls, as seen after ischemia and 15 minutes (p = 0.007; lanes 5 and 6) or ischemia and 4 hours of reperfusion (p = 0.04). Densitometry with relevant p values are shown beneath the gel. (IR = ischemia-reperfusion.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions

3. Fig 2
Electrophoretic mobility shift assay for signal transducer and activators of transcription-3 (STAT-3) nuclear translocation in whole lung homogenates. There is no baseline activation of STAT-3 in negative controls (lane 1), whereas ischemia followed by 15 minutes of reperfusion induced marked nuclear translocation of STAT-3 (lane 2), which was significant on densitometric analysis relative to negative control lungs (p = 0.01). As reperfusion progressed to the end of the experimental protocol at 4 hours of reperfusion, STAT-3 translocation diminished, but was still above baseline levels and significant relative to negative controls (p = 0.04; lane 4). The administration of intratracheal recombinant interleukin-6 (IL-6) protein reduced STAT-3 nuclear translocation significantly (p = 0.008) at 15 minutes (lane 3), but not at 4 hours (lane 5) of reperfusion relative to time-matched controls. Cold competition, as seen in lane 6, confirmed the band as STAT-3. Densitometry with relevant p values are shown beneath the gel. (IR = ischemia-reperfusion.)
The Annals of Thoracic Surgery  , DOI: ( /j.athoracsur )
Copyright © 2006 The Society of Thoracic Surgeons Terms and Conditions