1. Cryopreserved mouse hepatocytes retain regenerative capacity in vivo
Hyder Z. Jamal, Teresa C. Weglarz, Eric P. Sandgren 
Gastroenterology 
Volume 118, Issue 2, Pages (February 2000)
DOI: /S (00)
Copyright © 2000 American Gastroenterological Association Terms and Conditions

2. Fig. 1
(A and B) Sections through X-gal–stained recipient mouse livers from experiment 4. (A) Fresh cell recipient; (B) frozen/thawed cell recipient. Note that staining (dark gray to black) is localized to hepatocyte nuclei, and that the dark-staining donor hepatocyte clones are integrated into appropriately organized hepatic parenchyma (counterstained with nuclear fast red [light gray nuclei]; original magnification 200×). (C) BCIP-stained recipient mouse liver lobes from mice receiving 105 viable thawed hepatocytes that had been stored in liquid nitrogen for >32 months. Dark-stained areas illustrate the pattern and extent of clonal repopulation after splenic transfer of hepatocytes into MUP-uPA recipient mice. Unstained (near-white) areas of liver are composed of clones of endogenous cells that no longer express the transgene and therefore compete with donor cells during parenchymal repopulation.
Gastroenterology  , DOI: ( /S (00) )
Copyright © 2000 American Gastroenterological Association Terms and Conditions