1. Differential Expression of Matrix Metalloproteinases During Impaired Wound Healing of the Diabetes Mouse 
Steven J. Wall, Dr, Damon Bevan, David W. Thomas, Keith G. Harding, Dylan R. Edwards, Gillian Murphy 
Journal of Investigative Dermatology 
Volume 119, Issue 1, Pages (July 2002)
DOI: /j x
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Murine wound healing rates. The wound area was measured at the start and end of the wounding experiment and used to calculate the rate of wound closure (a). Tissue sections for each time point were also analyzed to determine the epidermal tongue area (b) and granulation tissue area (c). Each point represents the mean ± SEM. Significant differences are indicated by an asterisk (p <0.05). n = 10 for each point in (a) and n = 8 for each point in (b) and (c). Haemotoxylin and eosin stained tissue sections of nondiabetic (d) and diabetes(e) mouse day 10 wounds are also shown. Scale bar: 200 µm. a, adipose tissue; d, dermis; e, epidermis; gt, granulation tissue.
Journal of Investigative Dermatology  , 91-98DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
MMP-2 mRNA and protein expression profiles during wound repair. Real-time RT-PCR was performed on all samples and the MMP-2 mRNA time course was generated after the values had been normalized for GAPDH (a). Gelatin zymography was performed on all protein samples, quantified by 1D image analysis software and expressed as a percentage of the positive control. The wound tissue profile is shown in (b) and the wound fluid profile in (c). Each point represents the mean ± SEM. Significant differences are indicated by an asterisk (p <0.05). n = 6 for each point in (a) and n = 10 for each point in (b) and (c).
Journal of Investigative Dermatology  , 91-98DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
MMP-3 mRNA and protein expression profiles during wound repair. Real-time RT-PCR was performed on all samples and the MMP-3 mRNA time course was generated after the values had been normalized for GAPDH (a). Western blotting was performed on all protein samples, quantified by 1D image analysis software and expressed as a percentage of the positive control. The wound tissue profile is shown in (b) and the wound fluid profile in (c). Each point represents the mean ± SEM. Significant differences are indicated by an asterisk (p <0.05). n = 6 for each point in (a) and n = 10 for each point in (b) and (c).
Journal of Investigative Dermatology  , 91-98DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Localization of MMPs within the murine wound. Indirect immunofluorescence for MMP-3 and MMP-9 were performed on 10 µm tissue sections of diabetes and nondiabetic mouse wounds and unwounded skin. (a) Unwounded nondiabetic MMP-3 stained tissue section. Scale bar: 50 µm. (b) Day 1 nondiabetic MMP-3 stained tissue section. (c) Day 1 nondiabetic MMP-9 stained tissue section. (d) Day 3 nondiabetic MMP-9 stained tissue section. (e) Day 1 negative control. Arrow, tip of the epidermal tongue; green, positive FITC staining; blue, DAPI nuclear counter stain.
Journal of Investigative Dermatology  , 91-98DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
MMP-9 mRNA and protein expression profiles during wound repair. Real-time RT-PCR was performed on all samples and the MMP-9 mRNA time course was generated after the values had been normalized for GAPDH (a). Gelatin zymography was performed on all protein samples, quantified by 1D image analysis software and expressed as a percentage of the positive control. The wound tissue profile is shown in (b) and the wound fluid profile in (c). Each point represents the mean ± SEM. Significant differences are indicated by an asterisk (p <0.05). n = 6 for each point in (a) and n = 10 for each point in (b) and (c).
Journal of Investigative Dermatology  , 91-98DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

7. Figure 6
MMP expression within human acute and chronic wound fluid. Gelatin zymography (MMP-2 and MMP-9) and Western blotting (MMP-3) was performed on all protein samples, quantified by 1D image analysis software and expressed as a percentage of the positive control. MMP-2 expression levels are shown in (a), MMP-3 in (b), and MMP-9 in (c). Each point represents the mean ± SEM, where n = 13 for the chronic samples and n = 9 for the acute samples, with significant differences highlighted (*p <0.05).
Journal of Investigative Dermatology  , 91-98DOI: ( /j x)
Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions