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Volume 7, Issue 5, Pages (May 2003)

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Presentation on theme: "Volume 7, Issue 5, Pages (May 2003)"— Presentation transcript:

1 Volume 7, Issue 5, Pages 597-603 (May 2003)
Local adenoviral-mediated inducible nitric oxide synthase gene transfer inhibits neointimal formation in the porcine coronary stented model  Kai Wang, Paul D Kessler, Zhongmin Zhou, Marc S Penn, Farhad Forudi, Xiaorong Zhou, Khaldoun Tarakji, Melina Kibbe, Imre Kovesdi, Douglas E Brough, Eric J Topol, A.Michael Lincoff  Molecular Therapy  Volume 7, Issue 5, Pages (May 2003) DOI: /S (03) Copyright © 2003 The American Society of Gene Therapy Terms and Conditions

2 FIG. 1 Detection of iNOS expression in porcine coronary artery with RT-PCR after endoluminal gene transfer. Messenger RNA encoding human iNOS was detected in 6 of 8 Ad.hiNOS-transferred arteries, whereas no expression of iNOS was detected in the nontarget coronary arteries (LCX) or in a segment of the aorta. There was variability in gene transfer to the two arterial segments (animals 5 and 6) and between animals, with one animal (animal 7) showing no detectable iNOS in either artery. Molecular Therapy 2003 7, DOI: ( /S (03) ) Copyright © 2003 The American Society of Gene Therapy Terms and Conditions

3 FIG. 2 Photomicrographs of porcine coronary arteries 28 days after AdiNOS or AdNull transfer and stent implantation (Lawseon's elastic van Gieson staining, original magnification 2.5×). (A) Representative light microscopy cross section of stented coronary artery from AdNull group, showing extensive neointimal hyperplasia with smaller luminal area. (B) Representative light microscopy cross section of stented coronary artery from AdiNOS group, showing reduced neointimal hyperplasia with larger luminal area. L, lumen; N, neointima; M, media. Molecular Therapy 2003 7, DOI: ( /S (03) ) Copyright © 2003 The American Society of Gene Therapy Terms and Conditions

4 FIG. 3 Photomicrographs of cross sections of porcine coronary arteries 28 days after AdiNOS or AdNull transfer and stent implantation (hematoxylin and eosin staining, original magnification 10×). (A) Representative light microscopy cross section of stented coronary artery from AdNull group. (B) Representative light microscopy cross section of stented coronary artery AdiNOS group. There was no significant difference in mononuclear infiltrate present in the AdiNOS-treated vessel segment compared with AdNull-treated vessel segment. L, lumen; N, neointima; M, media; *stent strut. Molecular Therapy 2003 7, DOI: ( /S (03) ) Copyright © 2003 The American Society of Gene Therapy Terms and Conditions

5 FIG. 4 Photomicrographs of cross sections of porcine coronary arteries 28 days after AdiNOS or AdNull transfer and stent implantation (Masson's trichrome, original magnification 10×). (A) Representative light microscopy cross section of stented coronary artery from AdNull group. (B) Representative light microscopy cross section of stented coronary artery AdiNOS group. AdiNOS transfer significantly decreased extracellular matrix formation and migration, as evidenced by much weaker staining of extracellular matrix compared with AdNull-treated vessel segment. L, lumen; N, neointima; M, media; *stent strut. Molecular Therapy 2003 7, DOI: ( /S (03) ) Copyright © 2003 The American Society of Gene Therapy Terms and Conditions

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