1. Fig. 6. A circadian rhythm in keratinocyte wound healing and a diurnal variation in human burn healing outcome.
A circadian rhythm in keratinocyte wound healing and a diurnal variation in human burn healing outcome. (A) (i) Synchronized HaCaT monolayers expressing luciferase under control of the BMAL1 promoter (n = 24; mean ± SD) were wounded at the indicated times (vertical lines), and (ii) healing was monitored by confocal microscopy. (iii) Relative fluorescence in the wound area (n = 4; mean ± SEM) was calculated, and (iv) maximal healing after 15 hours was compared by Tukey’s multiple comparisons test (P values are indicated). (B) Mean time to 95% healing ± SEM from 118 human burn incidents separated by time of burn occurrence in 4-hour (left) or 12-hour (right) bins. ANOVA P value is indicated, as is the P value for Welch’s t test comparing daytime versus nighttime wounds. P values from Holm-Sidak’s test versus the 0000 to 0359 bin are indicated below. NA, not applicable.
Nathaniel P. Hoyle et al., Sci Transl Med 2017;9:eaal2774
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