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Development of Plasmid Calibrators for Absolute Quantification of miRNAs by Using Real-Time qPCR
Christine Formisano-Tréziny, Marina de San Feliciano, Jean Gabert The Journal of Molecular Diagnostics Volume 14, Issue 4, Pages (July 2012) DOI: /j.jmoldx Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions
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Figure 1 qPCR amplification of two independent hsa-let-7b plasmid calibrator ranges (A and B). Amplification plot (left panel) of 5 points (102, 103, 104, 105, and 106 copies) measured in duplicate. Calibration curve (right panel) with equation and R2 of the linear regression are indicated. The Journal of Molecular Diagnostics , DOI: ( /j.jmoldx ) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions
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Figure 2 Classical (thin line) and alternative (thick line) strategy workflow. The Journal of Molecular Diagnostics , DOI: ( /j.jmoldx ) Copyright © 2012 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions
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