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Combinatorial Enzymatic Synthesis of Heparan Sulfate

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Presentation on theme: "Combinatorial Enzymatic Synthesis of Heparan Sulfate"— Presentation transcript:

1 Combinatorial Enzymatic Synthesis of Heparan Sulfate
Robert J. Linhardt, Jin-Hwan Kim  Chemistry & Biology  Volume 14, Issue 9, Pages (September 2007) DOI: /j.chembiol Copyright © 2007 Elsevier Ltd Terms and Conditions

2 Figure 1 Chemoenzymatic Synthetic Scheme of Polysaccharide Variants of Heparan Sulfate from E. coli Heparosan NDST is N-deacetylase N-sulfotransferase; OSTs are O-sulfotransferases; and Epi is C5 epimerase catalyzing the conversion of glucuronic acid to iduronic acid. PAPS is required as a sulfate donor for NDST and OST enzymes. Chemistry & Biology  , DOI: ( /j.chembiol ) Copyright © 2007 Elsevier Ltd Terms and Conditions

3 Figure 2 Antithrombin Binding of Oligosaccharides and Full-Length Heparan Sulfate Polysaccharides as a Function of the Number of Saccharide Residues Chemistry & Biology  , DOI: ( /j.chembiol ) Copyright © 2007 Elsevier Ltd Terms and Conditions


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