1. Proteomic analysis of individual human embryos to identify novel biomarkers of development and viability 
Mandy G. Katz-Jaffe, Ph.D., David K. Gardner, Ph.D., William B. Schoolcraft, M.D. 
Fertility and Sterility 
Volume 85, Issue 1, Pages (January 2006)
DOI: /j.fertnstert
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1 The complexity of the human embryonic proteome.
Katz-Jaffe. Human embryo proteome. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Gel view of three individual expanded human blastocysts of similar morphology (designated as A, B, and C), highlighting the enhanced region of 4 kDa to 5 kDa.
Katz-Jaffe. Human embryo proteome. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
Box plot of normalized peak intensity for the 6.3-kDa up-regulated protein (P<.05) in expanded blastocysts compared with early blastocysts. Line represents mean.
Katz-Jaffe. Human embryo proteome. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
Negatively charged proteins showing significant differential expression related to blastocyst morphology. Open bars, degenerating embryos; solid bars, developing blastocysts. **Significantly different from developing blastocysts (P<.01).
Katz-Jaffe. Human embryo proteome. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

6. FIGURE 5
Scatter plot of normalized peak intensity for the 9.7-kDa up-regulated protein (P<.05) in degenerating embryos. Developing blastocysts include both the early and expanded groups. Line represents mean and dots represent each individual blastocyst. n = 5 and n = 16 for degenerating embryos and developing blastocysts, respectively.
Katz-Jaffe. Human embryo proteome. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions

7. FIGURE 6
Heat map segregating developing human blastocysts and degenerating human embryos according to protein profiles. Each column of squares represents an individual human embryo, whereas each row of squares represents an individual protein profile. Clustering analysis facilitates the grouping of similar protein expression profiles. Two major clusters were classified, as shown on the left of the heat map. The top cluster identified proteins that are up-regulated (red) in degenerating embryos, whereas the bottom cluster identified proteins that are up-regulated (red) in developing blastocysts. Red = up-regulated proteins; green = down-regulated proteins.
Katz-Jaffe. Human embryo proteome. Fertil Steril 2006.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2006 American Society for Reproductive Medicine Terms and Conditions