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Catherine I. Chardigny, MD, Victor A. Jebara, MD, Tony J

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Presentation on theme: "Catherine I. Chardigny, MD, Victor A. Jebara, MD, Tony J"— Presentation transcript:

1 Effects of cardioplegic solutions on the vasoreactivity of the internal mammary artery 
Catherine I. Chardigny, MD, Victor A. Jebara, MD, Tony J. Verbeuren, PhD, Alain F. Carpentier, MD, PhD, Jean-Noël Fabiani, MD  The Annals of Thoracic Surgery  Volume 66, Issue 2, Pages (August 1998) DOI: /S (98)

2 Fig 1 Organ bath apparatus with strain gauge transducer. (a = 20-mL water-jacketed glass organ bath; b = arterial ring segment mounted on two stainless steel wires; c = lower wire fixed to micrometer; d = upper wire attached to force transducer.) The Annals of Thoracic Surgery  , DOI: ( /S (98) )

3 Fig 2 (A) Drug protocol in Krebs solution and (B) protocol for incubation in cardioplegic solutions. (ACh = acetylcholine; KCl = potassium chloride; NE = norepinephrine; NPS = sodium nitroprusside.) The Annals of Thoracic Surgery  , DOI: ( /S (98) )

4 Fig 3 Dose-response curves to norepinephrine in Krebs solution before incubation in any cardioplegic solution. Sensitivity to norepinephrine was not significantly different between the 40 segments from the 10 patients. For clarity, standard errors of the mean for each point on each curve are not shown. (BC = blood cardioplegia; BH = Broussais Hospital solution; KCl = potassium chloride; NE = norepinephrine; UW = University of Wisconsin solution.) The Annals of Thoracic Surgery  , DOI: ( /S (98) )

5 Fig 4 Evolution of resting tension in University of Wisconsin solution. In ten segments (one from each patient) incubated in University of Wisconsin solution, a spontaneous contraction was observed for the first 5 minutes of incubation (2.11 ± 0.79 g), followed by a spontaneous decrease to resting tension within a mean of 5 minutes. The Annals of Thoracic Surgery  , DOI: ( /S (98) )

6 Fig 5 Dose-response curves to norepinephrine (NE) in Krebs solution after incubation in cardioplegic solutions. There was a significant difference in sensitivity to norepinephrine between segments in Krebs solution and segments in University of Wisconsin (UW) or Broussais Hospital (BH) solutions, but not segments incubated in blood cardioplegia (BC). For clarity, standard errors of the mean for each point on each curve are not shown. (KCl = potassium chloride.) The Annals of Thoracic Surgery  , DOI: ( /S (98) )

7 Fig 6 Endothelium-dependent relaxation to acetylcholine in Krebs solution after incubation in cardioplegic solutions. There was a significant difference in relaxation to acetylcholine between segments incubated in Krebs solution and those incubated in the three cardioplegic solutions and between segments incubated in blood cardioplegia (BC) and those incubated in University of Wisconsin solution (UW) or Broussais Hospital solution (BH). (NE = norepinephrine.) The Annals of Thoracic Surgery  , DOI: ( /S (98) )

8 Fig 7 Endothelium-independent relaxation to sodium nitroprusside in Krebs solution after incubation in cardioplegic solutions. There was no significant difference (p = NS) in percent relaxation to sodium nitroprusside between segments incubated in Krebs solution and in each cardioplegic solution. (BC = blood cardioplegia; BH = Broussais Hospital solution; NE = norepinephrine; UW = University of Wisconsin solution.) The Annals of Thoracic Surgery  , DOI: ( /S (98) )


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