1. Volume 131, Issue 5, Pages 1475-1485 (November 2006)
Eosinophilic Bowel Disease Controlled by the BB Rat-Derived Lymphopenia/Gimap5 Gene 
Lesley Cousins, Margaret Graham, Reuben Tooze, Christine Carter, J. Ross Miller, Fiona M. Powrie, Gordon G. Macpherson, Geoffrey W. Butcher 
Gastroenterology 
Volume 131, Issue 5, Pages (November 2006)
DOI: /j.gastro
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2. Figure 1
Pathology of PVG-RT1u, lyp/lyp rats. (A) Gross appearance of gastrointestinal tract lyp/lyp (Left) and WT (Right). (B and C) Cross sections of large intestine (original magnification, 100×). (B) WT, (C) lyp/lyp, and (D and E) cross sections of cecum (D) large intestine (E) of lyp/lyp rat (original magnification, 200×). (F and G) Sections of WT (F) and lyp/lyp small intestine (G). (H) Higher magnification of lyp/lyp small intestinal infiltrate (original magnification, 630×). (I and J) Alcian Blue staining of large intestine for goblet cells and mucus (original magnification, 200×) (I) lyp/lyp (J) WT. (K and L) Masson’s Trichrome staining for collagen (original magnification, 200×). (K) lyp/lyp large intestine and (L) WT large intestine. (B–H) H&E staining.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 AGA Institute Terms and Conditions

3. Figure 2
Eosinophils and mast cells in PVG-RT1u, lyp/lyp rats. (A) Large intestine stained for eosinophils by Chromotrope 2R (original magnification, 630×) (Left panel) WT, (Right panel) lyp/lyp. (B) Large intestine stained for the mast cell-specific protease RCMPII (brown) (L panel) WT, (R panel) lyp/lyp. Counterstain was Methyl Green (original magnification, 100×). (C) Eosinophil numbers in peripheral blood. Eosinophils were identified in flow cytometry by expression of CD172 and high side scatter signal. Data are presented as means ± SEM.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 AGA Institute Terms and Conditions

4. Figure 3
Cellular composition of secondary lymphoid organs of PVG-RT1u, lyp/lyp rats. (A) Total cell numbers; (B) total CD4+ T cells; (C) total CD8+ T cells; and (D) total B cell numbers in spleen, MLN, and superficial CLN of lyp/lyp rats and WT controls. (E) FACS plots showing lack of CD161+ TcRαβ+ NKT cells in the spleen of lyp/lyp rats. Data presented as mean ± SEM *P < .05, **P < .01.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 AGA Institute Terms and Conditions

5. Figure 4
Characterization of T cells in PVG-RT1u, lyp/lyp rats. (A) Analysis of surface marker expression by CD4+ T cells from MLN. MLN cells were harvested from 6-month PVG-RT1u, lyp/lyp, and control WT animals and labelled for FACS analysis. Cells gated for CD4 and αβTCR expression were analyzed for expression of CD45RC, OX-40, and CD25. (B) T-cell–derived cytokines. Splenocytes and lymph node cells were pooled from 4- to 6-month-old rats and sorted for CD4+ T cells and CD45RClo CD4+ T cells. Cells were then activated in vitro for 4 hours and secreted IL-4 and IFN-γ assayed by ELISA in supernatants. IL-5 and IL-13 mRNA expression was assayed in cell lysates by QPCR and normalized to β-actin expression. mRNA levels were calculated relative to those in PVG CD4+ T cells. Values are displayed as the mean of triplicates ± SD. Data are representative of 3 experiments.
Gastroenterology  , DOI: ( /j.gastro )
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6. Figure 5
B-cell responses in PVG-RT1u, lyp/lyp rats. (A) Serum IgG1, IgG2b, and IgE levels were measured in 6-month-old lyp/lyp rats and WT controls. (B) Time course of serum IgE levels in lyp/lyp rats. (C and D) Identification of IgE-positive B cells in MLN by costaining for CD45RA (green) and IgE (red) in lyp/lyp (C) and WT (D) rats. Original magnification 250×. (E and F) Labelling of IgE-positive (red) cells in the lamina propria of 6-month-old lyp/lyp rats (E) and WT (F) nuclear labelling with DAPI (original magnification, 400×).
Gastroenterology  , DOI: ( /j.gastro )
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7. Figure 6
Intestine-specific autoantibodies in PVG-RT1u, lyp/lyp rats. (A) Serum from lyp/lyp rats was incubated on intestinal cryostat sections from athymic nude (rnu/rnu) rats. Autoantibody was detected using anti-rat IgG secondary antibody. Nuclear staining is with DAPI (original magnification, 200×). (B) Higher magnification of lyp/lyp autoantibody staining (original magnification, 400×). (C) Intestinal autoantibody staining was not seen using WT serum (original magnification, 200×). (D) Double staining of lyp/lyp autoantibodies (green) and CD45 (red) showing no colocalization of autoantibody-positive cells and CD45+ cells. (E) lyp/lyp Serum was incubated with lung tissue. (F) Autoantibody staining using intestinal tissue derived from a germ-free Wistar rat.
Gastroenterology  , DOI: ( /j.gastro )
Copyright © 2006 AGA Institute Terms and Conditions