1. Role of vitamin C and selenium in attenuation of nicotine induced oxidative stress, P53 and Bcl2 expression in adult rat spleen 
Marwa A. Ahmed, K.H. Hassan, Khaled M.A. Hassanein, H. Waly 
Pathophysiology 
Volume 21, Issue 3, Pages (September 2014)
DOI: /j.pathophys
Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

2. Fig. 1
(a) Immunoblot detection (Western blot) showing changes in P53 levels in spleen tissues in different groups. Changes were probed using corresponding specific antibodies. (b) Changes in the mean values of P53 levels in spleen tissues for control and different groups. (c) Changes in the mean values of Bcl 2 levels in spleen tissues for all studied groups. Results are expressed as the mean±SE. P<0.001 for nicotine group versus control; P<0.01 for nicotine+Vit. C versus nicotine group; P<0.001 for nicotine+Se versus nicotine group.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

3. Fig. 2
The relative mRNA expression alterations of P53 were quantified in spleen tissues after different treatments using real-time quantitative RT-PCR. Means of three independent triplicates of the target genes were calculated normalized with their corresponding GAPDH values. ***p<0.001 for nicotine and nicotine+Se groups versus control group; NS: non significant versus control group.+++p<0.001 versus Nicotine +Se ###p<0.001 versus to nicotine group.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions

4. Fig. 3
The relative mRNA expression alterations of Bcl-2 were quantified in spleen tissues after different treatments using real-time quantitative RT-PCR. Means of three independent triplicates of the target genes were calculated, normalized with their corresponding GAPDH values. Data represented the average of three replicates with their standard. ***p<0.001; *p<0.05 versus control group. #p<0.05 versus N+Se group. NS: non significant; +p<0.05 compared to nicotine group.
Pathophysiology  , DOI: ( /j.pathophys )
Copyright © 2014 Elsevier Ireland Ltd Terms and Conditions