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Upregulation of lipocalin-2 (LCN2) in osteoarthritic cartilage is not necessary for cartilage destruction in mice  W.-S. Choi, J.-S. Chun  Osteoarthritis.

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Presentation on theme: "Upregulation of lipocalin-2 (LCN2) in osteoarthritic cartilage is not necessary for cartilage destruction in mice  W.-S. Choi, J.-S. Chun  Osteoarthritis."— Presentation transcript:

1 Upregulation of lipocalin-2 (LCN2) in osteoarthritic cartilage is not necessary for cartilage destruction in mice  W.-S. Choi, J.-S. Chun  Osteoarthritis and Cartilage  Volume 25, Issue 3, Pages (March 2017) DOI: /j.joca Copyright © 2016 The Author(s) Terms and Conditions

2 Fig. 1 LCN2 is upregulated in OA chondrocytes and cartilage. (A and B) LCN2 mRNA levels were detected by RT-PCR (A) and quantified by qRT-PCR (B) in chondrocytes stimulated with IL-1β (n = 5) and TNF-α (n = 5) or infected with the indicated MOIs of Ad-Epas1 (n = 7) and Ad-Zip8 (n = 7). (C) Representative images of alcian blue staining and immunostaining of LCN2 in damaged and undamaged regions of human OA cartilage (n = 6). (D) Representative images of safranin-O staining and immunostaining of LCN2 in cartilage sections from mice subjected to sham operation (n = 4), DMM surgery (n = 10), or IA injection with 1 × 109 plaque forming units of Ad-C (n = 10), Ad-Epas1 (n = 10) or Ad-Zip8 (n = 10). Values are the means ±95% CI. Scale bar: 50 μm. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2016 The Author(s) Terms and Conditions

3 Fig. 2 Upregulation of LCN2 is not sufficient or necessary for OA cartilage destruction in mice. (A) Protein and mRNA levels of LCN2 in chondrocytes infected with Ad-C (800 MOI) or the indicated MOI of Ad-Lcn2 (n = 6). (B–D) Mice were IA-injected with Ad-C or Ad-Lcn2 (1 × 109 PFU, once per week for 3 weeks), and sacrificed 3 or 8 weeks after the first injection. Representative images of immunohistochemical staining for LCN2 in the joint tissues (B; n = 10). OARSI grade and synovial inflammation (C; n = 10). Cartilage destruction and synovitis detected by safranin-O/hematoxylin staining (D; n = 10). (E) RT-PCR analysis of the indicated mRNAs in chondrocytes transfected with or without the Lcn2 siRNA and further treated with IL-1β (left; n = 5) or infected with 800 MOI of Ad-Epas1 (middle; n = 5) or Ad-Zip8 (right; n = 5). (F) Genotype and LCN2 mRNA levels in chondrocytes of WT and Lcn2 KO mice. (G) Cartilage sections from sham- and DMM-operated WT (n = 10) and Lcn2-KO mice (n = 10) were stained with safranin-O, and cartilage destruction was quantified by OARSI grade. Values are the means ±95% CI. Scale bar: 50 μm. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2016 The Author(s) Terms and Conditions

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