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Creating a heterozygous male
Male Parent genotype Female Parent genotype X Female gametes Male gametes Progeny genotype Progeny phenotypes and ratios Which progeny are you continuing with? Why?
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Linkage 1: Cross with Unlinked unc
Male Parent genotype +/+ ; unc/unc Female Parent genotype X Female gametes Male gametes F1 Progeny genotype F1 Progeny phenotypes and ratios
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Linkage 2: Selfing of hermaphrodite from unlinked cross
X self cross gametes Progeny genotype Progeny phenotypes and ratios
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Linkage 1: Crossing with Linked unc
Male Parent genotype + unc/ + unc Female Parent genotype X Female gametes Male gametes Progeny genotype Progeny phenotypes and ratios Which progeny are you continuing with? Why?
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Linkage 2: Selfing of hermaphrodite from linked cross
X self cross gametes Progeny genotype Progeny phenotypes and ratios
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Mapping 1: Making the double mutant
hermaphrodite X self cross gametes Progeny genotype Progeny phenotypes and ratios What if the genotype of the parent was + unc/+ unc?
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Mapping 2: Making heterozygotes for mapping
Male Parent genotype Female Parent genotype X Female gametes Male gametes Progeny genotype Progeny phenotypes and ratios Why might you see Dpy Unc progeny?
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Mapping 3: Test Cross Female Parent Male Parent genotype genotype X
Parental Recombinant Female gametes Male gametes Progeny genotype Progeny phenotypes and ratios
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Symbols to Know for Crosses
a//a – a is the allele and // represents the chromatids – there are two for a diploid organism – sometimes written as / a b//a b – two different genes on the same chromosome a//a ; b//b – two different genes on different chromosomes X – symbolizes mating between two different individuals X - symbolizes a self cross – when the hermaphrodite worms fertilize their own eggs
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