1. Confluent durable endothelialization of endarterectomized baboon aorta by early attachment of cultured endothelial cells 
Peter A. Schneider, MD, Stephen R. Hanson, PhD, Todd M. Price, BA, Laurence A. Harker, MD 
Journal of Vascular Surgery 
Volume 11, Issue 3, Pages (March 1990)
DOI: / (90)
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2. Fig. 1
The preparation of aortic segments. The vessel segment was cannulated at each end by Teflon tubing and secured with 3-0 silk ties. The vessel was then covered by an outer casing of Teflon to provide external support and prevent the possibility of blood being lost through the device.
Journal of Vascular Surgery  , DOI: ( / (90) )
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3. Fig. 2
Scanning electron microscopy of vessel preparations in vitro. A, The endarterectomized vascular surface. B, After incubation with cultured ECs for 20 minutes the endarterectomized surface displays attached, rounded, unspread ECs. C, EC spreading is complete after 2 hours perfusion with culture medium in vitro. D, The confluent endothelial monolayer is preserved during 1 hour exposure to highshear flow conditions in vitro. Scale bar is 10 μ.
Journal of Vascular Surgery  , DOI: ( / (90) )
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4. Fig. 3
Deposition of platelets on vascular segment preparations. Platelet deposition on endarterectomized segments was decreased after prior incubation with cultured ECs for 20 minutes in vitro. Untreated endarterectomized aortic segments were highly thrombogenic (upper curve). Platelet accumulation was negligible on the manipulated, nonendarterectomized aortic segments (lower curve).
Journal of Vascular Surgery  , DOI: ( / (90) )
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5. Fig. 4
Effects of EC attachment on thrombus formation in vivo. A, Surgical endarterectomy produces a flow surface of medial smooth muscle cells and connective tissue matrix. B, Incubation of the endarterectomy surface to cultured ECs for 20 minutes in vitro allows ECs to attach at saturation density. C, After exposure of untreated endarterectomized aortic segments to flowing blood for 1 hour, extensive platelet-rich thrombus forms. D, When EC-treated endarterectomized vessel is exposed to flowing blood under high shear (arterial) conditions for 1 hour, thrombus formation is markedly reduced, and the endothelial Cs undergo spreading to form a confluent monolayer. Scale bar is 10 μ.
Journal of Vascular Surgery  , DOI: ( / (90) )
Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions