1. GFP GAP-43 merge Vec Raf CAAX Raf S621A
Suppl.C
GFP
GAP-43
merge
Sustained activation of ERK pathway induces neurite outgrowth in TNB-1 cells
TNB-1 cells were transfected with the indicated pCDNA3-derived Raf constructs and the pEGFP-C3 reporter construct. Expression of activated Raf protein, RafCAAX, induced neurite outgrowth in TNB-1 cells.
RafCAAX : full-length Raf1 proteins containing　carboxyl-terminal fusions with the membrane localization sequences of Ki-ras.
RafS621A: dominant negative form of Raf1.
Vec
Raf
CAAX
Raf
S621A