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Jing Wen, MD, Peiyi Wang, MS, Sumona V. Smith, MD, Carolyn A

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Presentation on theme: "Jing Wen, MD, Peiyi Wang, MS, Sumona V. Smith, MD, Carolyn A"— Presentation transcript:

1 Syndecans are differentially expressed during the course of aortic aneurysm formation 
Jing Wen, MD, Peiyi Wang, MS, Sumona V. Smith, MD, Carolyn A. Haller, PhD, Elliot L. Chaikof, MD, PhD  Journal of Vascular Surgery  Volume 46, Issue 5, Pages (November 2007) DOI: /j.jvs Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

2 Fig 1 Immunohistochemical staining of the aorta of ApoE-/- mice prior to initiation of an atherogenic diet and infusion of angiotensin II. A, Syndecan-1, 100x, B, syndecan-2, 40x, and C, syndecan-4, 400x. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

3 Fig 2 Immunohistochemical staining of the abdominal aorta of ApoE-/- mice one week after initiation of an atherogenic diet and infusion of angiotensin II. A, Macrophage (arrowheads), B, neutrophil, C, syndecan-1 (arrowheads), D, syndecan-2, and E, syndecan-4. All images obtained at a magnification of 100x. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

4 Fig 3 Immunohistochemical staining of the abdominal aorta of ApoE-/- mice 1 week after initiation of an atherogenic diet and infusion of angiotensin II. A, Macrophage (arrowheads), B, neutrophil, C, syndecan-1 (arrowheads), and D, syndecan-4. All images obtained at a magnification of 200x. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

5 Fig 4 Immunohistochemical staining of the abdominal aorta in ApoE-/- mice four weeks after initiation of an atherogenic diet and infusion of angiotensin II. A, Macrophage 40x, B, macrophage 100x, C, neutrophil 40x (arrowhead), and D, neutrophil 100x (arrowhead). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

6 Fig 5 Immunohistochemical staining of the abdominal aorta of ApoE-/- mice 4 weeks after initiation of an atherogenic diet and infusion of angiotensin II. A, Syndecan-1 40x (arrowheads), B, syndecan-1 (arrowheads), 100x, C, syndecan-2, 40x, and D, syndecan-2, 100x (arrowhead, asterisk). Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

7 Fig 6 Immunohistochemical staining of the abdominal aorta of ApoE -/- mice four weeks after initiation of an atherogenic diet and infusion of angiotensin II. A, Macrophage, B, syndecan-1, and C, syndecan-4. All images were acquired at a magnification of 100x. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

8 Fig 7 Immunofluorescence staining of the abdominal aorta of ApoE-/- mice 4 weeks after initiation of an atherogenic diet and infusion of angiotensin II. A, Syndecan-1, B, macrophage, C, macrophage, syndecan-1, and DAPI, and D, macrophage, syndecan-1, and DAPI. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

9 Fig 8 Immunohistochemical staining of the abdominal aorta of ApoE-/- mice 8 weeks after initiation of an atherogenic diet and infusion of angiotensin II. A, Macrophage, B, syndecan-1, and C, syndecan-4. All images were acquired at a magnification of 100x. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

10 Fig 9 Immunohistochemical staining of the abdominal aorta of ApoE-/- mice 8 weeks after initiation of an atherogenic diet and infusion of angiotensin II. A, Macrophage (arrowheads), B, syndecan-1 (arrowheads), C, syndecan-4, and D, syndecan-2 (asterisk). All images were acquired at a magnification of 40x. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

11 Fig 10 Immunohistochemical staining of human abdominal aortic aneurysm wall. A, Verhoeff Van Gieson staining of human aortic wall (4x). B, Macrophage (anti-CD68), 20x. C, Syndecan-1, 20x. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

12 Fig 11 Immunohistochemical staining of human abdominal aortic aneurysm wall. A, Macrophage (anti-CD68), 20x. B, Syndecan-1, 20x. Journal of Vascular Surgery  , DOI: ( /j.jvs ) Copyright © 2007 The Society for Vascular Surgery Terms and Conditions

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