1. Effects of Butanedione Monoxime and Temperature on Prolonged Cardiac Storage 
Ji Zhang, MD, Robert D Furukawa, BSc, Stephen E Fremes, MD, Donald A.G Mickle, MD, Richard D Weisel, MD 
The Annals of Thoracic Surgery 
Volume 63, Issue 2, Pages (February 1997)
DOI: /S (96)

2. Fig. 1
The recovery of developed pressure (mean ± standard deviation, n = 6/group) was enhanced in the BDM groups in comparision with unmodified UWS hearts. Zero to 8°C were the preferred storage temperatures in the BDM group and 4°C with unmodified UWS. (BDM = University of Wisconsin solution supplemented with 2,3-butanedione monoxime 10 mmol/L and calcium 0.1 mmol/L; UWS = University of Wisconsin solution.)
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

3. Fig. 2
Representative individual left ventricular end-diastolic pressure–volume curves are depicted before storage (Pre-storage) and after 12 hours of storage at 4°C (Post-storage). The upper panel was obtained using University of Wisconsin solution supplemented with 10 mmol/L 2,3-butanedione monoxime and 0.1 mmol/L calcium (BDM), and the lower panel was acquired using unmodified University of Wisconsin solution (UWS). The left ventricular end-diastolic pressure–volume curve was shifted upward and to the left (increased slope and decreased x-intercept) with unmodified University of Wisconsin solution yet minimally affected with BDM.
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

4. Fig. 3
Diastolic volume at a left ventricular end-diastolic pressure of 20 mm Hg is presented as a percentage of the prestorage value (mean ± standard deviation, n = 6/group). Recovery of diastolic function was improved with University of Wisconsin solution (UWS) supplemented with 10 mmol/L 2,3-butanedione monoxime and 0.1 mmol/L calcium (BDM) for temperatures 8°C or less. The optimal storage temperature was 0° to 8°C with BDM and 4° to 8°C with unmodified University of Wisconsin solution.
The Annals of Thoracic Surgery  , DOI: ( /S (96) )

5. Fig. 4
Poststorage coronary flow (mean ± standard deviation, n = 6/group) was greater with University of Wisconsin solution (UWS) supplemented with 10 mmol/L 2,3-butanedione monoxime and 0.1 mmol/L calcium (BDM) versus unmodified University of Wisconsin solution (UWS) for storage at 0°, 4°, and 8°C. Results for the BDM groups were significantly greater at 0° to 8°C than at 12° to 16°C. There was no significant temperature effect detected in the UWS hearts.
The Annals of Thoracic Surgery  , DOI: ( /S (96) )